In this study, to choose a sutiable bioreactor type for the mass culture of Phellinus linteus mycelium, we cultured Phellinus linteus mycelium using a stirred tank fermenter and an airlift fermenter and compared the performances of the two fermenters. The effects of aeration rate and agitation speed on the culture of Phellinus linteus mycelium were also investigated in the ranges of 1-4L/min, 200-300rpm, respectively. For the batch submerged culture, the dry weight of mycelium, pH, and dissolved oxygen concentration changed in four steps, respectively. But the periods of same steps were not consistent with each other. With an increase in aeration rate, the final dry weight of mycelium, dissolved oxygen concentration and pH value until the third step of pH change were increased. As the time increased, the concentration of glucose decreased. However, the effects of aeration rate and agitation speed on the variation of glucose concentration were negligible. The maximum final dry weight of mycelium was obtained when agitation speed was 300rpm. The dissolved oxygen concentration was increased with agitation speed. The dry weight of mycelium and dissolved oxygen concentration in the continuous stirred tank fermenter were larger than them those in the airlift fermenter.