Korean Chemical Engineering Research, Vol.54, No.6, 822-829, 2016
Enhanced Production of Cellulase-Free Thermoactive Xylanase Using Corncob by a Black Yeast, Aureobasidium pullulans CBS 135684
Our aim was to optimize the production of cellulase-free thermoactive xylanase by Aureobasidium pullulans CBS 135684 with statistical methodology based on experimental designs. Among eleven variables, the nutrient sources that had significant effect on xylanase production were corncob, (NH4)2SO4, xylose, KH2PO4 and tween 80, identified by the initial screening method of Plackett-Burman. The optimum concentrations of these five components were subsequently investigated using response surface methodology. The optimal concentrations (g·l-1) for maximum production of xylanase were corncob, 39.0; (NH4)2SO4, 3.0; xylose, 1.8; KH2PO4 1.4; and tween 80, 1.4, respectively. An improved xylanase yield of 8.74 ± 0.84 U·ml-1 was obtained with optimized medium which is 2.1-fold higher production than previously obtained results (4.10 ± 0.10 U·ml-1) after 48 h of cultivation. In addition, the xylanase production under optimal condition reached 10.09 ± 0.27 U·ml-1 after 72 h of cultivation.
[References]
Beg QK, Kapoor M, Mahajan L, Hoondal GS, Appl. Microbiol. Biotechnol. , 56 (3-4), 326, 2001
Viikari L, Alapuranen M, Puranen T, Vehmaanpera J, Siika-Aho M, Adv. Biochem. Eng. Biotechnol. , 108 , 121, 2007
Viikari L, Poutanen K, Tenkanen M, Tolan JS, “Hemicellulases,” Encyclopedia of Bioprocess Technology. Wiley, New York, (2002).
Woldesenbet F, Gupta N, Sharma P, Arch. Appl. Sci. Res. , 4 , 524, 2012
Bankeeree W, Lotrakul P, Prasongsuk S, Chaiareekij S, Eveleigh DE, Kim SW, Punnapayak H, Springerplus , 3 (1), 37, 2014
Gangwar AK, Prakash NT, Prakash R, Bioresources , 9 (2), 3733, 2014
Benedetti ACEP, Costa ED, Aragon CC, Santos AF, Goulart AJ, Attili-Angelis D, Monti R, Rev. Cienc. Farm. Basica Apl. , 34 (1), 25, 2013
Silva CJSM, Roberto IC, Process Biochem. , 36 (11), 1119, 2001
Rao KJ, Kim CH, Rhee SK, Process Biochem. , 35 (7), 639, 2000
Plackett RL, Burman JP, Biometrika , 33 (4), 305, 1946
Li Y, Liu ZQ, Cui FJ, Xu YY, Zhao H, J. Food Sci. , 72 (5), 320, 2007
Cui F, Liu Z, Li Y, Ping L, Ping L, Zhang Z, Lin L, Dong Y, Huang D, Biotechnol. Bioprocess Eng. , 15 (2), 299, 2010
Atlas RM, “Handbook of Microbiological Media,” L. C. Boca Raton, Florida (1993).
Leathers TD, J. Ind. Microbiol. Biotechnol. , 4 (5), 341, 1989
Miller GL, Anal. Chem. , 31 (3), 426, 1959
Box GE, Behnken DW, Technometrics , 2 (4), 455, 1960
Aro N, Ilmen M, Saloheimo A, Penttila M, Appl. Environ. Microbiol. , 69 (1), 56, 2003
Margolles-Clark E, Ihnen M, Penttila M, Biotechnol. J. , 57 (1), 167, 1997
Kulkarni N, Shendye A, Rao M, Fems Microbiol. Rev. , 23 (4), 411, 1999
Li Y, Lin J, Meng D, Lu J, Gu G, Mao Z, Food Technol. Biotech. , 44 (4), 473, 2006
Dobberstein J, Emeis CC, Appl. Microbiol. Biotechnol. , 32 (3), 262, 1989
Dobberstein J, Emeis CC, Appl. Microbiol. Biotechnol. , 32 (3), 262, 1989
Karni M, Deopurkar RL, Rale VB, World J. Microb. Biot. , 9 (4), 476, 1993
Reese ET, Maguire A, J. Appl. Microbiol. , 17 (2), 242, 1969
Shi JG, Zeng GM, Yuan XZ, Dai F, Liu J, Wu XH, World J. Microb. Biot. , 22 (11), 1121, 2006
Ding CH, Jiang ZQ, Li XT, Li LT, Kusakabe I, World J. Microb. Biot. , 20 (1), 7, 2004
Shah AR, Madamwar D, Process Biochem. , 40 (5), 1763, 2005
Maalej I, Belhaj I, Masmoudi NF, Belghith H, Appl. Biochem. Biotechnol. , 158 (1), 200, 2009
Nasr S, Soudi MR, Salmanian AH, Ghadam P, Iran J. Basic. Med. Sci. , 16 (12), 1245, 2013
Chen Y, Guo J, Li F, Liu M, Zhang X, Guo X, Xiao D, Biotechnol. Bioprocess Eng. , 19 (2), 282, 2014
Michelin M, Maria de Lourdes TM, Ruzene DS, Silva DP, Ruiz HA, Vicente AA, Jorge JA, Terenzi HF, Teixeira JA, Bioprocess. Biosyst. Eng. , 35 (7), 1185, 2012
Jin N, Ma S, Liu Y, Yi X, He R, Xu H, Qiao DR, Cao Y, Afr. J. Microbiol. Res. , 6 (10), 2387, 2012
Shah AR, Madamwar D, Process Biochem. , 40 (5), 1763, 2005
Pal A, Khanum F, Food Technol. Biotech. , 49 (2), 228, 2011
이전 논문 다음 논문
Result Search