The moment analysis of lysozyme was implemented using chromatograms that were obtained from weak cation exchange column in high performance liquid chromatography system. Three elution sodium phosphate buffers containing 1.0, 0.75, 0.5M sodium chloride were used. Experiments were conducted by varying flow rate, elution sodium chloride concentration, and lysozyme solute concentration. The general rate (GR) model was employed to calculate the first moment and the second moment. By plotting L/u0 vs. (μ1-t0)/(1-εe)(1-εi)] equilibrium constants (K) were obtained from first moment analysis. Intra-particle diffusivity was obtained from theoretical plate number data. Based on the results of moment analysis, van Deemter plots were drawn in order to investigate the contributions of Hax, Hf, and Hd to total Height Equivalent to a Theoretical Plate (HETP, Htotal). The effect of intra-particle diffusion (Hd) was the most dominant factor contributing to HETP while external mass transfer (Hf) was negligible factor.