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Publication history
Received January 30, 2023
Revised February 14, 2023
Accepted February 16, 2023
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Biosorption of Methylene Blue from Aqueous Solution using Dried Rhodotorula glutinis Biomass

1School of Liberal Arts and Education, University of Seoul, Seoul 02504, Korea 2Department of Chemical and Biological Engineering, Inha Technical College, Incheon 22212, Korea 3Department of Chemical Engineering, University of Seoul, Seoul 02504, Korea
Korean Chemical Engineering Research, May 2023, 61(2), 273-277(5), 10.9713/kcer.2023.61.2.273 Epub 31 May 2023
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The biosorption of methylene blue (MB) from aqueous solution was investigated using dried Rhodotorula glutinis as the biosorbent. The effects of pH, initial dye concentration, biosorbent dosage, and kinetic studies were determined to obtain valuable information for biosorption. Results indicated that most of the adsorbed MB bound within 30 minutes of contact and the MB adsorption capacity increased from 21.1 to 101.8 mg/g with the initial MB concentration increased from 50 to 300 mg/L. Additionally, the MB adsorption capacity gradually increased from pH 4.0 to 9.0, reaching its peak at an initial pH of 9.0. As the biomass load was increased from 0.25 to 4.0 g/L, the MB removal efficiency increased from 14.1 to 84.5%. The Langmuir model provided the best fit throughout the concentration range, and the maximum adsorption capacity (qmax) and Langmuir constant (b) were determined to be 135.14 mg/g and 0.026 l/mg, respectively. Furthermore, the biosorbent process of R. glutinis was found to follow pseudo-second-order kinetics and the calculated qeq,cal value showed good agreement with the experimental qeq value. Overall, the biosorption of MB by R. glutinis can be characterized as a monolayer, single site type phenomenon, and the rate-limiting step was determined to be the chemical reaction between the adsorbent and the adsorbate.


2. Saratale, G. D., Saratale, R. G., Chang, J. S. and Govindwar, S.P., “Fixed-bed Decolorization of Reactive Blue 172 by Proteus vulgaris NCIM-2027 Immobilized on Luffa Cylindrica Sponge,”Int. Biodeterior. Biodegrad., 65, 494-503(2011).
3. Hunger, K., Industrial dyes: Chemistry, properties and applications, Willey-VCH, Weinheim (2003).
4. Kishor, R., Saratale, G. D., Saratale, R. G., Ferreira, L. F. R., Bilal,M., Iqbal, H. M. and Bharagava, R. N., “Efficient Degradation and Detoxification of Methylene Blue Dye by a Newly Isolated Ligninolytic Enzyme Producing Bacterium Bacillus Albus MW407057,”Colloids Surfaces B Biointerfaces, 206, 111947(2021).
5. Khan, I., Saeed, K., Zekker, I., Zhang, B., Hendi, A. H., Ahmad,A., Ahmad, S., Zada, N., Ahmad, H., Shah, L. A., Shah, T. and Khan, I. “Review on Methylene Blue: Its Properties, Uses, Toxicity and Photodegradation,” Water, 14(2), 242(2022).
6. Aksu, Z., Ertuğrul, S. and Dönmez, Z., “Methylene Blue Biosorption by Rhizopus arrhizus: Effect of SDS (sodium dodecylsulfate)Surfactant on Biosorption Properties,” Chem. Eng. J., 158(3), 474-481(2010).
7. Silva, F., Nascimento, L., Brito, M., da Silva, K., Paschoal, W.Jr. and Fujiyama, R., “Biosorption of Methylene Blue Dye Using Natural Biosorbents Made from Weeds,” Materials (Basel), 12(15),2486(2019).
8. Cherifa, F., Hakima, C., Radhia, Y., Salah, H. and Razika K.,“Eosin Biosorption from Aqueous Solution on Two Types of Activated Sludge,” Kor. Chem. Eng. Res., 60(1), 80-85(2022).
9. Cho, D. H. and Kim, E. Y., “Characterization of Pb2+ Biosorption from Aqueous Solution by Rhodotorula glutinis”, Bioprocess Biosyst. Eng., 25, 271-277(2003).
10. Bai, J., Li, Z., Fan, F. Wu, X., Tian, W., Yin, X., Liang, Z., Fan,F., Tian, L., Wang, Y., Qin, Z. and Guo, J., “Biosorption of Uranium by Immobilized Cells of Rhodotorula glutinis”, J. Radioanal. Nucl.Chem., 299, 1517-1524(2014).
11. Saravanan, P., Sivacumar, P., Princy, G., Nagendra, G. and Renganathan, S., “Biosorption of Acid Green 1 Using Dried Rhodoturula glutinis Biomass,” Indian J. Environ. Protect., 32, 207-214(2012).
12. Langmuir, I., “The Adsorption of Gases on Plane Surface of Glass, Mica and Platinum,” J. Am. Chem. Soc., 40,1361-1368(1916).
13. Freundlich, H. M. F., “Over the Adsorption in Solution,” J. Phys.Chem., 57, 385-470(1906).
14. Lagergren, S., “About the Theory of so-called Adsorption of Soluble Substances,” Kung. Sven. Veten Hand., 24, 1-39(1989).
15. Ho, Y. S. and McKay, G., “Pseudo-second Order Model for Sorption Processes,” Process Biochem., 34, 451-465(1999).
16. Miraboutalebi, S. M., Nikouzad, S. K., Peydayesh, M., Allahgholi,N., Vafajoo, L. and McKay, G., “Methylene Blue Adsorption via Maize Silk Powder: Kinetics, Equilibrium, Thermodynamic Studies and Residual Error Analysis,” Process Saf. Environ. Prot.106, 191-202(2017).
17. Hameed, B. H., “Spent Tea Leaves: a New Non-conventional and Low-cost Adsorbent for Removal of Basic Dye from Aqueous Solutions,” J. Hazard. Mater. 161, 753-759(2009).
18. Jacob, J., Rabago, S., Ramos, R. L., Utrilla, J. R., Perez, R. O. and Cordova, F. J. C., “Biosorption Mechanism of Methylene Blue from
Aqueous Solution on to White Pine (Pinus durangensis) Sawdust:Effect of Operation Condition,” Sus. Environ. Res. 27, 32-40(2017).
19. Cho, D. H., Chu, K. H. and Kim, E. Y., “Loss of Cell Components During Rehydration of Dried Rhodotorula glutinis and Its
Implications for Lead Uptake,” Eng. Life Sci., 11(3), 283-290(2011).
20. Silva F., Nascimento, L., Brito, M., Silva, K., Paschoal, W. and Fujiyama, R., “Biosorption of Methylene Blue Dye Using Natural Biosorbents Made from Weed,” Materials. 12, 2486-2501(2019).
21. Tural B., Ertas, E., Enez, B., Fincan, S. A. and Tural, S., “Preparation and Characterization of a Novel Magnetic Biosorbent Functionalized with Biomass of Bacillus Subtilis: Kinetic and Isotherm Studies of Biosorption Processes in the Removal of Methylene Blue,” J. Environ. Chem. Eng., 5, 4795-480(2017).
22. Iqbal, A., Sabar, S., Mun-Yee, M. K., Nur Asshifa, M. N., Yahya,A. R. M. and Adam, F., “Pseudomonas aeruginosa USM-AR2/SiO2 Biosorbent for the Adsorption of Methylene Blue,” J. Environ. Chemical Eng., 6(4), 4908-4916(2018).
23. Chowdhury, S. and Saha, P. D., “Biosorption of Methylene Blue from Aqueous Solutions by Waste Biomaterial: Hen Feathers,”Appl. Water Sci., 2, 209-219(2012).

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