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Language: korean

Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received November 4, 2015
Accepted January 9, 2016
Available online June 14, 2016

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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NaOH를 이용한 우드칩의 당화 전처리에 대한 감마선 조사 영향 연구

Effect of Gamma Irradiation on Wood Chip Saccharification Pretreated with NaOH

김수미 최종일^† 조민호¹ 김종덕²

Su-mi Kim Jong Jong-il Choi^† Min-Ho Joe¹ Jong-deog Kim²

전남대학교 생물공학과 바이오에너지및바이오소재 협동과정, 61186 광주광역시 북구 용봉로 77 ¹한국원자력연구원 첨단방사선연구소, 56212 전북 정읍시 금구길 29 ²전남대학교 생명산업공학과, 59626 전남 여수시 대학로 50

Department of Biotechnology and Bioengineering, Interdisciplinary Program for Bioenergy & Biomaterials, Chonnam National University, 77, Yongbong-ro, Buk-gu, Gwangju, 61186, Korea ¹Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 29, Geumgu-gil, Jeongeup, Jeonbuk, 56212, Korea ²Department of Biotechnology, Chonnam National University, 50, Daehak-ro, Yeosu, Chonnam, 59626, Korea

choiji01@chonnam.ac.kr

Korean Chemical Engineering Research, June 2016, 54(3), 431-435(5)
https://doi.org/10.9713/kcer.2016.54.3.431

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Abstract

본 논문에서는 감마선 조사가 NaOH 전처리를 이용한 우드칩의 당화에 미치는 영향을 살펴보았다. 당화량은 전처리된 우드칩에 효소를 이용하여 가수분해한 후 생성된 환원당과 포도당 양을 측정하여 확인하였다. 10 g/L NaOH 처리한 우드칩을 각각 50, 100, 200 kGy 세기의 감마선을 조사하여 당화량을 측정하였다. 우드칩의 가수분해 수율을 비교했을 때, 200 kGy 감마선 조사한 우드칩에서 가장 높은 환원당 함량 12.2 g/L이 얻어졌다. 또한 전처리 과정에서의 감마선 조사 단계의 영향을 평가하기 위하여 우드칩을 먼저 50, 100, 200 kGy 감마선 조사한 후, 10 g/L NaOH 로 전처리하였다. 200 kGy 감마선 조사 후 NaOH 전처리를 수행했을 때 환원당 함량은 13.4 g/L로 전처리 이후의 감마선 조사구보다 증가하였고, 7.9 g/L 포도당을 얻을 수 있었다. 이러한 결과로부터 감마선 조사가 셀루로오즈 바이오매스 전처리에 활용 가능할 것으로 기대된다.

The aim of this study was to investigate the effect of gamma irradiation on the pretreatment of wood chips with NaOH solution. The degree of saccharification was quantified by measuring reducing sugar and glucose concentrations after enzymatic hydrolysis. After pretreatment with 10 g/L NaOH, the wood chips were irradiated at the doses of 0, 50, 100, and 200 kGy, respectively. Among the irradiated samples, wood chips irradiated at the dose of 200 kGy had the highest reducing sugar concentration of 12.2 g/L. Also, to define the effect of irradiation before pretreatment, the wood chips were first gammairradiated and then pretreated with NaOH. When the NaOH treatment was conducted after irradiation at 200 kGy, the reducing sugar content was further increased to 13.4 g/L and glucose content of the wood chip was as high as 7.9 g/L. These results suggest that gamma irradiation may be the promising method for pretreatment of cellulose biomass.

Keywords

NaOH Pretreatment Gamma irradiation Wood chip

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