We investigated the thermal stability of polyphenols and fatty acids from Laminaria japonica powder by subcritical water hydrolysis among the range of the experimental conditions. The experiment was carried out at temperatures and pressures ranging from 200 to 280 ℃ and 13 to 60 bar, respectively for 28 to 42 min. Polyphenol and fatty acids in hydrolysates obtained from different conditions were analyzed by GC and UV-spectrophotometer. The results were compared with those obtained from Laminaria japonica oils extracted by supercritical CO2. Polyphenol and several fatty acids in hydrolysates produced by subcritical water showed high thermal stability.
Determination of substances characteristic of green and black tea.Part 1: Content of total polyphenols in tea, Colorimetric method using Folin-Ciocalteu reagent, ISO 145021-1:, 2005
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Andersson T, Parameters affecting the extraction of polycylic aromatic hydrocarbons with pressurized hot water, Academic dissertation, University of Helsinki, Finland, 2007