A single sugar α-linked glucuronic acid based oligosaccharide (SSGO) is water soluble oligosaccharides (WSOS) obtained by Gluconacetobacter hansenii PJK (KCTC 10505BP) as a byproduct during bacterial cellulose (BC) production. In this study, SSGO was used for the improvement of BC production by the vinegar bacterium, Acetobacter xylinum, which produces heteropolysaccharides as a byproduct. The addition of 1.0% SSGO to the chemically defined medium (CDM) resulted in an 89.3% increase in BC production by A. xylinum after 15 days of cultivation under static condition, and a 52.3% increase in BC production by G. hansenii. Both the dry cell weight and live cell density of A. xylinum increased 50% with the addition of 1.0% SSGO. SSGO successfully improved BC production by A. xylinum.