It is important to understand the differences in characteristics between plasmid-harboring recombinant yeast and chromosome-integrated recombinant yeast for the design and optimization of bioprocess employing recombi-nant yeast. In the present study, heterologous glucoamylase gene was inserted into yeast. The glucoamylase activity per gene copy number of chromosome-integrated recombinant yeast MMY2SUCSTA-I-5 was 3 to 6 folds higher than that of plasmid-harboring recombinant yeast MMY2SUCSTA. And the genetic stability of chromosome-integrated recombinant yeast (99%) was far better compared to plasmid-harboring recombinant yeast (65%). Better genetic stability and glucoamylase activity per gene copy number of chromosome-integrated recombinant yeast can provide advantages in higher final expression level, especially in continuous culture, compared to the plasmid-harboring recombinant yeast. The optimal glucose concentration for maximum expression of glucoamylase in chromosome-integrated recombinant yeast was lower than that in plasmid-harboring recombinant yeast.