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Issue
Korean Journal of Chemical Engineering,
Vol.25, No.6, 1439-1443, 2008
Fluorometric analysis on physicochemical properties of phosphatidylcholine-based W/O microemulsion involved with enzymatic reactivity in phospholipid hydrolysis
Yamazaki K, Imai M
Abstract.Interfacial tension and fluorometric analysis were employed for the investigation of the interfacial local fluidity and the hydrophobicity of the micro water pool in the PC-based water-in-oil (W/O) microemulsion. These microenvironment properties strongly influenced the phospholipase A2 reactivity for phospholipid hydrolysis in the W/O microemulsion. The organic phase was prepared by mixing of isooctane as a main solvent and 1-butanol as a co-solvent. The critical micelle concentration (CMC) was dramatically decayed from 9mM to 0.025mM by the increasing of the 1-butanol content. The local interfacial fluidity of the micro water pool was measured by using fluorescence polarity indicated by 1,6-Diphenyl-1,3,5-hexatriene-4'-trimethylammonium tosylate (TMA-DPH) and Coumarin 343 (C343). It was apparently increased with increasing the molar ratio of additive 1-butanol. In contrast, the hydrophobicity of the water pool measured by C343 was almost constant throughout the molar ratio of additive 1-butanol. Additive alcohol influenced the micro fluidity and enhanced reactivity of phospholipase A2 in lipid hydrolysis.
Keywords: W/O Microemulsion; Phospholipid; Hydrophobicity; Interfacial Fluidity; Fluorometric Analysis
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