A novel chitosan/nano-TiO2 composite emulsion (CTCE) was prepared by inverse suspension technology, and its multi-function properties were studied. As can be seen from the results, the gauze treated with chitosan/nano-TiO2 composite emulsion showed excellent antibacterial activities against Escherichia coli, Aspergillus niger and Candida albicans that the bactericidal ratios could reach 99.96%, 100% and 78.3% after 24 h, respectively. At the same time, the chitosan/nano-TiO2 composite emulsion could really kill the bacteria, not just inhibit its growth, and the bactericidal ratio for E. coli could reach 70.3% after 1 h. The bactericidal ratio for E. coli reached 98.8% at 27 ℃ compared with 58.7% at 18 ℃ after 8 h due to the activity of bacteria being weakened at low temperature. Furthermore, the antibacterial ability of antibacterial gauze was hardly influenced with the increase of storage time, which could be reused for up to 8 times without loss of antibacterial ability.