Print: | ISSN 0256-1115 |
Online: | ISSN 1975-7220 |
Korean Journal of Chemical Engineering,
Vol.25, No.5, 1125-1130, 2008 Analysis and comparison of cloning methods for the preparation of repetitive polypeptides
Repetitive polypeptides, defined as a protein consisting primarily of tandemly repeated blocks of amino acid sequence, are widely used biomaterials. These repetitive polypeptides can be used in diverse biological fields including tissue engineering scaffolds, drug delivery systems, biomaterials, and DNA separation systems. The physical/chemical properties of the repetitive polypeptides can be improved by changing the composition of the repeated amino acid sequence. In this study, we introduced genetic methods for the production of repetitive polypeptides. By using recursive directional ligation (RDL) and controlled cloning method (CCM), multimerized genes were cloned and identified. Also, we compared the characteristics of recursive directional ligation (RDL) with those of controlled cloning method (CCM).
Keywords:
Repetitive Polypeptide; ELP; Recursive Directional Ligation (RDL); Controlled Cloning Method (CCM)
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