The usage of ultrasound has gained popularity in the recent years. Ultrasonic processing means blasting liquids, usually water, with very intense sound at high frequency, producing very well mixed powerful chemical and physical reactions. This paper deals with the investigation of the effect of ultrasonic energy on the conversion fraction of sulfur in the production reaction of sodium thiosulfate which was obtained from sulfur and Na2SO3 solution. Concentration of Na2SO3 solution parameter was chosen as the constant parameter. The experiments were performed with various amplitudes of ultrasound power and in the absence and presence of ultrasound energy in various temperatures with various particle sizes. The results indicate that the conversion fraction values increase in the presence of ultrasound energy and increase at high amplitude values.