This study investigated the production behavior of amino acids from polypeptone under various operation parameters and reactor modes (batch and continuous). In the batch experiments, the effect of heating time on amino acid production was studied using different type of reactor materials (Hastelloy (HAS) and SUS). The results showed that the production behavior of amino acids such as Gly, Ala, and Leu was quite similar regardless of reactor materials. To further investigate the relationship of reaction time on the amino acids production, experiments were conducted in batch and continuous reaction at the same conditions (T=523 K and P=30MPa). The results showed that it is possible to get the same result at the same reaction time using a batch and continuous reaction mode if the reaction time is started after heating time in a batch reaction mode. From the study of reaction pressure, it was observed that the production of amino acids is not favored at reaction pressure between 4 and 30 MPa at 523 K.