Korean Journal of Chemical Engineering, Vol.22, No.3, 412-417, 2005
Characteristics of the Lipase from Candida rugosa Modified with Copolymers of Polyoxyethylene Derivative and Maleic Acid Anhydride
The hydrophilic copolymer, polyethylene oxide (PEO) allyl ester-maleic anhydride (MA), copolymer was used to modify the lipase from Candida rugosa. MA, in a functional group, reacts easily with amino acids of lipase. The degree of modification (DM) was varied by changing the weight ratio of copolymer to protein of lipase over the range of 10-120 (w/w). The specific activity decreased as DM increased. At the maximum modification degree of 35%, the modified lipase retained more than 65% of the unmodified native lipase activity. The modified lipase displayed a high stability of activity against temperature and pH. The remaining activity of modified lipase was about 2-4 fold of that of native lipase in the severe pH and temperature condition. Finally, it showed 20% greater reaction of substrate at 10 hr than in the case where native lipase was used.
[References]
Benjamin S, Pandey A, Yeast , 14 , 1069, 1998
Borgstrom B, Brockman HL, Lipases, Elsevier, New York, 1984
Bousquet MP, Willemot RM, Monsan P, Boures E, J. Biotechnol. , 68 , 61, 1999
Chae HJ, In MJ, Kim EY, Appl. Biochem. Biotechnol. , 69 , 53, 1998
Charusheela A, Arvind L, Enzyme Microb. Technol. , 30 (1), 19, 2002
Diamond AD, Hsu JT, Advances in Biochem. Eng. Biotech. , 49 , 89, 1992
Dordick JS, Biocatalysis for Industry, Plenum Press, New York, 1991
Dossat V, Combes D, Marty A, J. Biotechnol. , 97 , 117, 2002
Eijsink VGH, Bjork A, Gaseidnes S, Sirevag R, Synstad B, Burg B, vandenVriend G, J. Biotechnol. , 113 , 105, 2004
Furukawa M, Kodera Y, Uemura T, Hiroto M, Matsushima A, Kuno H, Matsushita H, Inada Y, Biochem. Biophys. Res. Commun. , 199 , 41, 1996
Goto M, Kamiya N, Miyata M, Nakashio F, Biotechnol. Prog. , 10 (3), 263, 1994
Guo Z, Sun Y, Biotechnol. Prog. , 20 (2), 500, 2004
Habeeb AFSF, Anal. Biochem. , 14 , 328, 1966
Jaeger K, Reetz M, Trends Biotechnol. , 16 , 396, 1998
Jeon GJ, Hur BK, Yang JW, Korean J. Biotechnol. Bioeng. , 14 , 696, 1999
Jensen RG, Lipids , 18 , 650, 1983
Joo H, Yoo YJ, Dordick JS, Korean J. Chem. Eng. , 15 (4), 362, 1998
Gee KB, Choi CY, Korean J. Chem. Eng. , 1 (1), 13, 1984
Kim Y, Park K, Lee H, Jang S, Song HC, Shin HC, Park JJ, Park J, Maken S, J. Ind. Eng. Chem. , 10 (3), 384, 2004
Koeller KM, Wong CH, Nature , 409 , 232, 2001
Kontkanen H, Tenkanen M, Fagerstrom R, Reinikainen T, J. Biotechnol. , 108 , 51, 2004
Kwon DY, Rhee JS, Korean J. Chem. Eng. , 1 (2), 153, 1984
Lee SK, Park SW, Kim YI, Chung KH, Hong SI, Kim SW, Korean J. Chem. Eng. , 19 (2), 261, 2002
Longo MA, Combes D, J. Chem. Technol. Biotechnol. , 74 (1), 25, 1999
Matsumoto M, Kida K, Kondo K, J. Chem. Technol. Biotechnol. , 76 (10), 1070, 2001
Moon HY, Kim SY, Park JW, Kajiuchi T, Korean J. Chem. Eng. , 10 (4), 235, 1993
Nishio T, Takahashi K, Tsuzuki T, Yoshimoto T, Kodera Y, Matsushima A, Saito Y, Inada Y, J. Biotechnol. , 8 , 39, 1988
Noel M, Combes D, J. Biotechnol. , 102 , 23, 2003
Park CY, Ryu YW, Kim C, Korean J. Chem. Eng. , 18 (4), 475, 2001
Park KN, Park JW, J. Appl. Polym. Sci. , 77 (2), 368, 2000
Park JW, Park KN, Biotechnol. Tech. , 13 , 49, 1999
Park JW, Korean J. Chem. Eng. , 12 (5), 523, 1995
Park J, Park K, Bioresour. Technol. , 79 (1), 91, 2001
Park JW, Park KN, Song HC, Shin HC, J. Biotechnol. , 93 , 203, 2002
Park K, Park J, Song H, Shin H, Park J, Ahn JS, Korean J. Chem. Eng. , 19 (2), 285, 2002
Park SW, Kim YI, Chang KS, Kim SW, Process Biochem. , 37-2 , 153, 2001
Reetz MT, Curr. Opin. Chem. Biol. , 6 , 145, 2002
Schmid A, Dordick JS, Hauer B, Wubbolts M, Witholt B, Nature , 409 , 258, 2001
Wu JC, Song BD, Process Biochem. , 37 , 1229, 2002
Wu XY, Jaaskelainen S, Linko YY, Enzyme Microb. Technol. , 19 (3), 226, 1996
Wu JC, He ZM, Yao CY, Yu KT, J. Chem. Technol. Biotechnol. , 76 (9), 949, 2001
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