A two-section simulated moving bed (SMB) modified from a three-section SMB [Barker et al., 1975] was applied to the separation of an aqueous mixture of glucose and fructose at high concentration up to 500 kg/m(3). Dowex 50W-X12 resin of Ca(++) form was used as an adsorbent and water as an isocratic eluent. The equilibrium isotherms in terms of a quadratic expression and a plug flow model with mass transfer effect were used to predict both the products and on-concentrations in the two-section SMB process. The two-section SMB process suggested in this work was successful in obtaining high fructose corn syrup (fructose with 55-90% w/w) at the high concentration of 500 kg/m(3).