Bacterial L-asparaginase has been widely used as a potential therapeutic agent in the treatment of various lymphoblastic leukemia diseases. We studied product and dual substrates utilization kinetics by P. carotovorum MTCC 1428 in batch bioreactor. The kinetic study revealed that the maximum growth of P. carotovorum MTCC 1428 was achieved at 2 g l-1 and 5 g l-1 of glucose and L-asparagine, respectively. Different substrate inhibition models were fitted to the growth kinetic data and the additive form of double Luong model was found to best explain the growth kinetics of P. carotovorum MTCC 1428. The kinetic parameters of growth studies showed that the predicted maximum inhibition concentration of glucose (Smg) and L-asparagine (Sma) was close to the experimentally observed value 15.0 and 10 gl-1, respectively. Modified form of the Luedeking-Piret model was used to describe the kinetics of L-asparaginase production, and the system seems to be mixed growth associated. Kinetic models of dual substrate growth, L-asparaginase production and substrate(s) utilization by P. carotovorum MTCC 1428 well fitted with experimental data with regression coefficients (R2) value of 0.97, 0.96 and 0.93, respectively.