We investigated the effect of temperature on the efficiency of paclitaxel extraction from biomass. In addition, kinetic and thermodynamic studies of this extraction process were performed. The concentration of extracted paclitaxel increased with increasing extraction temperature and extraction time. When the experimental data were applied to various kinetic models, the hyperbolic model (second-order model) was the most appropriate. The predictive model was developed to predict the concentration of extracted paclitaxel at different temperatures at a given time. The Gibbs free energy change was determined to be negative, while enthalpy change and entropy change were positive. These results indicate that this extraction process is spontaneous, endothermic, and irreversible.