A facile and rapid method for the synthesis of porous gold (PAu) electrodes on cellulose-filters is proposed. Morphology and active surface area of the electrode greatly influence the electrochemical properties of sensor. Compared to smooth electrode and glassy carbon, PAu and Pt/PAu electrode showed high roughness due to porous structure, which is helpful in electrochemical sensing of target molecules. The electrochemical sensing performance of Pt/PAu electrode was evaluated to detect hydrogen peroxide, glucose, and perchloric acid. The amperometric results with various concentrations of target molecules showed potential applications of Pt/PAu electrode in sensitive and effective chemical sensors.