Korean Journal of Chemical Engineering, Vol.31, No.1, 98-103, 2014
Immobilization of Candida sp.99-125 lipase onto silanized SBA-15 mesoporous materials by physical adsorption
SBA-15-NH2 and SBA-15-Cl Mesoporous Materials were prepared by modifying SBA-15 with silane coupling agent 3-aminopropyltriethoxysilane and 3-chloropropyltriethoxysilane using the post-synthesis method. The mesoporous samples were characterized by Fourier transform infrared spectra and nitrogen adsorption. Compared with SBA-15-NH2, SBA-15-Cl has suitable pore opening for further utilization in the immobilization of Candida sp.99-125 lipase by physical adsorption. The influences of lipase concentration and immobilizing time on the immobilization efficiency were investigated. Meanwhile, the lipase immobilized on SBA-15-Cl showed higher thermal, pH and storage stability than that of free lipase. Further study demonstrated that lipase immobilized on SBA-15-Cl could be used eight times without significant decrease of enzyme activity. The phenomenon was associated with the shrinkage of the pore opening of SBA-15-Cl keeping lipase from leaping out.
[References]
Jaeger KE, Dijkstra BW, Reetz MT, Annu. Rev. Microbiol. , 53 , 315, 1999
Li Y, Zhou G, Li C, Qin D, Qiao W, Chu B, Colloid Surface A. , 341 , 79, 2009
Salis A, Meloni D, Ligas S, Casula MF, Monduzzi M, Solinas V, Dumitriu E, Langmuir , 21 (12), 5511, 2005
Vinu A, Murugesan V, Tangermann O, Hartmann M, Chem.Mater. , 16 , 3056, 2004
Galarneau A, Mureseanu M, Atger S, Renard G, Fajula F, New J. Chem. , 30 , 562, 2006
Zhang ST, Gao SF, Gao GQ, Appl. Biochem. Biotechnol. , 160 (5), 1386, 2010
Tischer W, Wedekind F, Top. Curr. Chem. , 200 , 95, 1999
Krajewska B, Enzyme Microb. Technol. , 35 (2-3), 126, 2004
Zou B, Hu Y, Yu D, Jiang L, Liu W, Song P, Colloids Surf.,B. , 88 , 93, 2011
Rodrigues DS, Mendes AA, Adriano WS, Goncalves LRB, Giordano RLC, J. Mol. Catal. B-Enzym. , 51 , 100, 2008
Bayramoglu G, Karagoz B, Altintas B, Arica MY, Bicak N, Bioprocess. Biosyst. Eng. , 34 , 735, 2011
Wang X, Dou P, Zhao P, Zhao C, Ding Y, Xu P, ChemSus-Chem. , 2 , 947, 2009
Lee D, Ponvel KM, Kim M, Hwang S, Ahn I, Lee C, J. Mol.Catal. B-Enzym. , 57 , 62, 2009
Bayramoglu G, Hazer B, Altintas B, Arica MY, Process. Biochem. , 46 , 372, 2011
Arica MY, Soydogan H, Bayramoglu G, Bioprocess. Biosyst. Eng. , 33 , 227, 2010
Karagoz B, Bayramoglu G, Altintas B, Bicak N, Arica MY, Ind. Eng. Chem. Res. , 49 (20), 9655, 2010
Lee J, Kim J, Kim J, Jia H, Kim M, Kwak JH, Jin S, Dohnalkova A, Park HG, Chang HN, Wang P, Grate JW, Hyeon T, Small. , 1 , 744, 2005
Hartmann M, Chem. Mater. , 17 , 4577, 2005
Il Kim M, Kim J, Lee J, Jia H, Bin Na H, Youn JK, Kwak JH, Dohnalkova A, Grate JW, Wang P, Hyeon T, Park HG, Change HN, Biotechnol. Bioeng. , 96 (2), 210, 2007
Li Y, Zhou G, Qiao W, Wang Y, Mater. Sci. Eng. B-Adv. , 16 , 120, 2009
Serra E, Mayoral A, Sakamoto Y, Blanco RM, Diaz I, Micropor. Mesopor. Mater. , 114 , 201, 2008
Forde J, Vakurov A, Gibson TD, Millner P, Whelehan M, Marison IW, Fagain CO, J. Mol. Catal. B-Enzym. , 66 , 203, 2010
Ikemoto H, Chi Q, Ulstrup J, J. Phys. Chem. C. , 114 , 16174, 2010
Jaladi H, Katiyar A, Thiel SW, Guliants VV, Pinto NG, Chem. Eng. Sci. , 64 (7), 1474, 2009
Pallavi S, Sridevi N, Prabune A, Ramasamy V, The 40th Anniversary of International Zeolite Conference, 1891, 2007
Yiu HHP, Wright PA, Botting NP, J. Mol. Catal. B-Enzym. , 15 , 81, 2001
Ye P, Jiang J, Xu Z, Colloids Surf., B. , 60 , 62, 2007
Wang X, Chan JCC, Tseng Y, Cheng S, Micropor. Mesopor. Mater. , 95 , 57, 2006
Kang Y, He J, Guo XD, Guo X, Song ZH, Ind. Eng. Chem. Res. , 46 (13), 4474, 2007
Abdullah AZ, Sulaiman NS, Kamaruddin AH, Biochem.Eng. J. , 44 , 263, 2009
Zhu K, Wang J, Wang Y, Liu H, Han P, Wei P, J. Nanosci.Nanotechnol. , 11 , 7593, 2011
Wang J, Huang L, Xue M, Wang Y, Gao L, Zhu JH, Zou Z, J. Phys. Chem. C. , 112 , 5014, 2008
Serri NA, Kamaruddin AH, Long WS, Bioprocess. Biosyst.Eng. , 29 , 253, 2006
Bradford MM, Anal. Biochem. , 72 , 248, 1976
Fuentes IE, Viseras CA, Ubiali D, Terreni M, Alcantara AR, J. Mol. Catal. B-Enzym. , 11 , 657, 2001
Llusar M, Monros G, Roux C, Pozzo JL, Sanchez C, J. Mater.Chem. , 13 , 2505, 2003
Adam F, Osman H, Hello KM, J. Colloid Interface Sci. , 331 (1), 143, 2009
Zhao G, Wang J, Li Y, Chen X, Liu Y, J. Phys. Chem. C. , 115 , 6350, 2011
Katiyar A, Ji L, Smirniotis P, Pinto NG, J. Chromatogr. A. , 1069 , 119, 2005
Wang CF, Zhou GW, Xu YQ, Chen J, J. Phys. Chem. C. , 115 , 22191, 2011
Bayramoglu G, Kiralp S, Yilmaz M, Toppare L, ArIca MY, Biochem. Eng. J. , 38 , 180, 2008
Bayramoglu G, YIlmaz M, Senel AU, ArIca MY, Biochem.Eng. J. , 40 , 262, 2008
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