The malate-pyruvate conversion pathway is catalyzed by two malic enzyme isomers, MaeA and MaeB. qRT-PCR was carried out under malate and pyruvate supplemented conditions to understand the dynamics of maeA and maeB gene expression. maeA expression was elevated by malate, and maeB expression was inhibited by levels of both malate and pyruvate higher than 0.1 mM. Green fluorescent protein (GFP) reporter plasmids were also constructed by integration of the maeA/maeB promoter with the gfp gene. We showed that maeA driven GFP expression
was positively and negatively correlated with extracellular malate and pyruvate induction. In contrast, no significant changes in maeB driven GFP expression were observed under both malate and pyruvate supplemented conditions.
Wang B, Wang P, Zheng E, Chen X, Zhao H, Song P, Su R, Li X, Zhu G, J. Microbiol., 49, 797, 2011
Miller WG, Leveau JHJ, Lindow SE, Mol. Plant Microbe In., 13, 1243, 2000
Sambrook J, Russell DW, Molecular cloning: A laboratory manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1, 2001
Eleaume H, Jabbouri S, J. Microbiol. Methods., 59, 363, 2004
Moon SY, Hong SH, Kim TY, Lee SY, Biochem. Eng. J., 40, 312, 2008
Zelle RM, de Hulster E, van Winden WA, de Waard P, Dijkema C, Winkler AA, Geertman JMA, van Dijken JP, Pronk JT, van Maris AJA, Appl. Environ. Microbiol., 74, 2766, 2008