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Korean Journal of Chemical Engineering, Vol.30, No.9, 1775-1783, 2013
Chitosan effects on glass matrices evaluated by biomaterial. MAS-NMR and biological investigations
Oudadesse H, Wers E, Bui XV, Roiland C, Bureau B, Akhiyat I, Mostafa A, Chaair H, Benhayoune H, Faure J, Pellen-Mussi P
Bioactive glass 46S6 and biodegradable therapeutic polymer (Chitosan: CH) have been elaborated to form 46S6-CH composite by freeze-drying process. The kinetics of chemical reactivity and bioactivity at the surface were investigated by using physicochemical techniques, particularly solid-state MAS-NMR. Immortalized cell line used to construct multicellular spheroids was employed as three-dimensional (3D) cell cultures for in vitro studies. Obtained results showed a novel structure of the composite; the chemical treatment (ultrasound, magnetic stirring, freeze drying process and lyophilization) led the bioactive glass particles to be loaded in the chitosan-based materials. 29Si and 31P MAS-NMR results showed the emergence of two new species, QSi3(OH) and QSi4, which are characteristic of the vitreous network dissolution in simulated body fluid (SBF). MAS-NMR also confirmed the formation of amorphous calcium
phosphate (ACP) at the surface of the initial 46S6-CH. Three-dimensional (3D) cell cultures highlighted the effect of chitosan, where the cell viability reached up to 78% in 46S6-CH composite and up to 67% in 46S6. The association of (CH) and bioactive glass (BG) matrix promotes a highly significant bioactivity, demonstrating surface bone formation and satisfactory behavior in biological environment.
[References]
- Hench LL, Splinter RJ, Allen WC, Greenlee TK, J.Biomed. Mater. Res., 5, 117, 1971
- Hench LL, J. Sci. Mater: Mater. Med., 17, 967, 2006
- Rich J, Jaakkola T, Tirri T, Narhi T, Yli-Urpo A, Seppala C, J. Biomater., 23, 2143, 2002
- Walsh D, Furuzono T, Tanaka A, J. Biomater., 22, 1205, 2001
- Devin J, Attawia M, Laurencin C, J. Sci. Biomater. Polym. Edn., 7, 661, 1996
- Leonor IB, Baran ET, Kawashita M, Reis RL, Kokubo T, Nakamura T, Acta Biomater., 4, 1349, 2008
- Thein-Han WW, Misra RDK, Acta Biomater., 5, 1182, 2008
- Brandenberg G, Leibrock LG, Shuman R, Malette WG, Neurosur., 15, 9, 1984
- Muzzarelli RA, Tanfani F, Emanuelli M, Pace DP, Chiumzzi E, Res. Carbohyd., 126, 225, 1984
- Hirano S, Yagi Y, Res. Carbohyd., 8, 103, 1980
- Jiang L, Li Y, Wang X, Zhang L, Wen J, Gong M, Carbohyd.Poly., 74, 680, 2008
- Zhao L, Chang J, J. Sci. Mater: Mater. in Med., 15, 625, 2004
- Joel Bumgardner D et al., US Patent, 0254007 A1, 2007
- Mathew P, Binulal NS, Soumya S, Nair SV, Furuike T, Tamura H. Jayakumar R, Carbohyd. Poly., 79, 281, 2009
- Boyan BD, et al., US Patent, 5,492,697, 1996
- Dietrich E, Oudadesse H, Lucas-Girot A, Mami M, J. Biomed.Mater. Res. A., 88, 1087, 2008
- Bui XV, Oudadesse H, Gal YL, Merdrignac-Conanec O, Cathelineau G, Korean J. Chem. Eng., 29(2), 215, 2012
- Bui XV, PhD, Elaboration de biomateriaux verres-substances actives (Zoledronate-Chitosan), Order Number 4421, University of Rennes 1, France, 171, 21 November 2011.
- Kokubo T, Kushitani H, Sakka S, Kitsugi T, Yamamuro T, J.Biomed. Mater. Res., 24, 721, 1990
- Massiot D, Fayon F, Capron M, King I, Le Calve S, Alonso B, Durand JO, Bujoli B, Gan Z, Hoaston G, Magn. Reson.Chem., 40, 70, 2002
- Lockyer MWG, Holland D, Dupree R, J. Non. Cryst. Solids., 188, 207, 1995
- Elgayar I, Aliev AE, Boccaccini AR, Hill RG, J. Non. Cryst.Solids., 351, 173, 2005
- Angelopoulou A, Montouillout V, Massiot D, Kordas G, J.Non. Cryst. Solids., 354, 333, 2008
- Hinedi ZR, Goldberg S, Chang AC, Yesinowski JP, J. Colloid Interface Sci., 152, 141, 1992
- Belton PS, Harris RK, Wilkes PJ, J. Phys. Chem. Solids., 49, 21, 1988
- Aue WP, Roufosse AH, Glimcher MJ, Griffin RG, Biochem., 23, 6110, 1984
- Lin KSK, Tseng TH, Mou Y, Hsu YC, Yang CM, Chan JCC, Chem. Mater., 17, 4493, 2005
- Philips NG, YHLo AY, Isabel IB, Ana G, Daniel A, Baltzar S, Jekabs G, Maria VR, Mattias E, J. Phys. Chem. C., 114, 9345, 2010
- Diaz A, Lopez T, Manjarrez J, Basaldella E, Martinez-Blanes JM, Odriozola JA, Acta Biomater., 2, 173, 2006
- Oudadesse H, Dietrich E, Bui XV, Le Gal Y, Pellen P, Cathelineau G, Appl. Surf. Sci., 257(20), 8587, 2011
- Machenzie KJD, Smith ME, Multinuclear solid-state NMR of inorganic materials, Cambridge: Pergamon Mater Series, 2002
- Oestrike R, Yang WH, Kirkpatrick RJ, Hervig RL, Navrotsky A, Montez B, Geochem. Cosmochim Acta., 51, 2199, 1987
- Oudadesse H, USTV, October 2011, Rennes, France, 2011
- Anderson OH, Karlsson KH, J. Non. Cryst. Solids., 129, 145, 1991
- Alno N, Jegoux F, Pellen-Mussi P, Tricot S, Oudadesse H, Cathelineau G, De Mello G, Biomed GJ, Mater. Res. A., DOI:10.1002/jbm.a.32818.
- Dietrich E, PhD, Syntheses et etudes physic-chimiques de verres bioactifs denses et poreux, Order Number 3767, University of Rennes 1, France, 205, 17 October 2008.
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