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Korean Journal of Chemical Engineering, Vol.34, No.11, 2901-2915, 2017
Enhanced production of glutaminase free L-asparaginase II by Bacillus subtilis WB800N through media optimization
We studied the crucial components which elevate the expression of recombinant novel glutaminase free Lasparaginase II (rL-asp II) from Bacillus subtilis WB800N. The Plackett-Burman tool identified sucrose, NH4Cl, NaH2- PO4 and MgSO4 as the significant influencing factors (p<0.05). Further investigations showed that artificial neural network- genetic algorithm (ANN-GA) was more effective than central composite design (CCD) in optimizing the influencing factors. The maximum rL-asp II expression was found to be 389.56 IU/ml and 525.98 IU/ml using CCD (R2=90.4%) and ANN-GA (R2=96.2%), respectively. The validation experiments were carried out in a 3 L batch bioreactor where kinetic modelling of the obtained data was done. The rL-asp II expressed effectively inhibiting the polyacrylamide formation in vitro where no solidification was observed, when 2ml of purified rL-asp II used even after 60 min of incubation. This is the first study to report highest production of rL-asp II in B. subtilis WB800N (525.98 IU/ ml) till date by combining statistical designs with consecutive intermittent addition of IPTG in batch reactor.
[References]
- Altenbern RA, Housewright RD, Arch. Biochem. Biophys., 49, 130, 1954
- Broome JD, J. Natl. Cancer Inst., 35, 967, 1965
- Borek D, Jaskolski M, Acta Biochim. Pol., 48, 893, 2001
- Peterson RE, Ciegler A, Appl. Microbiol. Biotechnol., 17, 929, 1969
- Holmquist ND, Proc. Soc. Exp. Biol. Med., 113, 444, 1963
- Michalska K, Bujacz G, Jaskolski M, J. Mol. Biol., 360, 105, 2006
- Grover CE, Chibnall AC, Biochem. J., 21, 857, 1927
- El-Bessoumy AA, Sarhan M, Mansour J, J. Biochem. Mol. Biol., 37, 387, 2004
- Lopes AM, Oliveira-Nascimento L, Ribeiro A, Tairum CA, Breyer CA, Oliveira MA, Monteiro G, Aouza-Motta CM, Magalhaes PO, Avendano JG, Cavaco-Paulo AM, Mazzola PG, Rangel-Yagui CO, Sette LD, Converti A, Pessoa A, Crit. Rev. Biotechnol., 37, 82, 2017
- Tong WH, Pieters R, Kaspers GJL, Loo DMWMT, Bierings MB, Bos CVD, Kollen WJW, Hop WCJ, Lanvers-Kaminsky C, Relling MV, Tissing WJE, Sluis IMVD, Blood, 123, 2026, 2014
- UmaMaheswari T, Hemalatha T, Sankaranarayanan P, Puvanakrishnan R, Indian J. Exp. Biol., 54, 7, 2016
- Schwartz JH, Reeves JY, Broome JD, Proc. Natl. Acad. Sci. U.S.A., 56, 1516, 1966
- Wriston JC, Yellin TO, Adv. Enzymol. Relat. Areas Mol. Biol., 39, 185, 1973
- Verma N, Kumar K, Kaur G, Anand S, Crit. Rev. Biotechnol., 27, 45, 2007
- Sajitha S, Vidya J, Varsha K, Binod P, Pandey A, Biochem. Eng. J., 102, 14, 2015
- Jain S, Naithani R, Kapoor G, Nath T, Leuk. Res., 33, 194, 2009
- Reinert RB, Oberle LM, Wek SA, Bunpo P, Wang XP, Mileva I, Goodwin LO, Aldrich CI, Durden DL, McNurlan MA, Wek RC, Anthony TG, J. Biol. Chem., 281, 31222, 2016
- Chityala S, Dasu VV, Ahmad J, Prakasham RS, Bioprocess. Biosyst. Eng., 38, 2271, 2015
- Pedreschi F, Mariotti S, Granby K, Risum J, LWT - Food Sci. Technol., 44, 1473, 2011
- Rytting MM, Blood Lymphat. Cancer Targets Ther, 2, 117, 2012
- Labrou NE, Muharram MM, Enzyme Microb. Technol., 92, 86, 2016
- Ciesarova Z, Kiss E, Boegl P, J. Food Nutr. Res., 45, 141, 2006
- Ciesarova Z, Kukurova K, Benesova C, Nutr. Food Sci., 40, 50, 2010
- Pedreschi F, Kaack K, Granby K, Food Chem., 109, 386, 2008
- Tajner-Czopek ERA, Miedzianka J, Kita A, Kita A, Nems A, Hamouz K, Int. J. Food Prop., NULL, 1, 2017
- Macauley-Patrick S, Fazenda ML, McNeil B, Harvey LM, Yeast, 22, 249, 2005
- Rosano GL, Ceccarelli EA, Front. Microbiol., 5, 172, 2014
- Onishi Y, Yano S, Thongsanit J, Takagi K, Yoshimune K, Wakayama M, Ann. Microbiol., 61, 517, 2011
- Xie J, Zhao Y, Zhang H, Liu Z, Lu Z, Lett. Appl. Microbiol., 58, 53, 2014
- Sarvas M, Harwood CR, Bron S, van Dijl JM, Biochim. Biophys. Acta, 1694, 311, 2004
- Jung J, Yu KO, Ramzi AB, Choe SH, Kim SW, Han SO, Biotechnol. Bioeng., 109(9), 2349, 2012
- Oh C, De Zoysa M, Kang DH, Lee Y, Whang I, Nikapitiya C, Heo SJ, Yoon KT, Affan A, Lee J, J. Microbiol. Biotechnol., 21, 1021, 2011
- Kamada M, Sumitaka H, Kazushi F, Masato M, Kengo S, Keitarou K, Yasubumi S, PloS One, 10, 014136, 2015
- Palva I, Gene, 19, 81, 1982
- Simonen M, Palva I, Microbiol. Rev., 57, 109, 1993
- Nguyen TT, Quyen TD, Le HT, Microb. Cell Factories, 12, 79, 2013
- Luo ZC, Gao QQ, Li XL, Bao J, Appl. Biochem. Biotechnol., 173(2), 562, 2014
- Cayuela C, Kai K, Park YS, Iijima S, Kobayashi T, J. Ferment. Bioeng., 75, 383, 1993
- Park YS, Kai K, Iijima S, Kobayashi T, Biotechnol. Bioeng., 40, 686, 1992
- Vuolanto A, von Weymarn N, Kerovuo J, Ojamo H, Leisola M, Biotechnol. Lett., 23(10), 761, 2001
- Matsui H, Sato K, Enei H, Shibai H, Hirose Y, Agric. Biol. Chem., 43, 1325, 1979
- Gu XB, Zheng ZM, Yu HQ, Wang J, Liang FL, Liu RL, Process Biochem., 40(10), 3196, 2005
- Khan AW, Rahman MS, Zohora US, Okanami M, Ano T, J. Environ. Sci. China, 23, 63, 2011
- Westers L, Dijkstra DS, Westers H, van Dijl JM, Quax WJ, J. Biotechnol., 123, 211, 2006
- Wenzel M, Muller A, Siemann-Herzberg M, Altenbuchner J, Appl. Environ. Microbiol., 77, 6419, 2011
- Dasu VV, Panda T, Bioprocess Eng., 22, 45, 2000
- Reddy LVA, Wee YJ, Yun JS, Ryu HW, Bioresour. Technol., 99(7), 2242, 2008
- Myers RH, Montgomery DC, Anderson-Cook CM, John Wiley & Sons (2016).
- Pal MP, Vaidya BK, Desai KM, Joshi RM, Nene SN, Kulkarni BD, J. Ind. Microbiol. Biotechnol., 36, 747, 2009
- Prabhu AA, Jayadeep A, Prep. Biochem. Biotechnol., 47, 397, 2016
- Michalewicz Z, Genetic Algorithms+Data Structures=Evolution Programs, Springer Berlin Heidelberg (1996).
- Bryjak J, Ciesielski K, Zbiciriski I, J. Biotechnol., 114, 177, 2004
- Luli GW, Strohl WR, Appl. Environ. Microbiol., 56, 1004, 1990
- Snay J, Jeong JW, Ataai MM, Biotechnol. Prog., 5, 63, 1989
- Plackett RL, Burman JP, Biometrika, 33, 305, 1946
- Wacker I, Ludwig H, Reif I, Blencke HM, Detsch C, Stulke J, Microbiology, 149, 3001, 2003
- Cornell JAK, Response surfaces: designs and analyses, Marcel Dekker, Inc., New York (1987).
- Araujo PW, Brereton RG, Trends Anal. Chem., 15, 63, 1996
- Kumar S, Pakshirajan K, Dasu VV, Appl. Microbiol. Biotechnol., 84(3), 477, 2009
- Desai KM, Survase SA, Saudagar PS, Lele SS, Singhal RS, Biochem. Eng. J., 41, 266, 2008
- Khayet M, Cojocaru C, Sep. Purif. Technol., 86, 171, 2012
- Yasin Y, Ahmad FBH, Ghaffari-Moghaddam M, Khajeh M, Environ. Nanotechnol. Monit. Manag., 1, 2, 2014
- Gera N, Uppaluri RVS, Sen S, Venkata Dasu V, Chem. Biochem. Eng. Q., 22, 315, 2008
- Guo Y, Lou F, Peng ZY, Yuan ZY, Korus RA, Biotechnol. Bioeng., 35, 99, 1990
- Ornelas AP, Silveira WB, Sampaio FC, Passos FML, J. Appl. Microbiol., 104(4), 1008, 2008
- Amrane A, Adour L, Couriol C, Biochem. Eng. J., 24, 125, 2005
- Saat MN, Annuar MSM, Alias Z, Chuan LT, Chisti Y, Bioprocess. Biosyst. Eng., 37, 765, 2014
- Rajendran A, Thangavelu V, J. Bioprocess. Biotech., 2, 118, 2012
- Singh Y, Srivastava SK, J. Chem. Technol. Biotechnol., 89(1), 117, 2014
- Meena B, Anburajan L, Dheenan PS, Begum M, Vinith Kumar NV, Dharani G, Kirubagaran R, Bioprocess. Biosyst. Eng., 38, 373, 2015
- Bradford MM, Anal. Biochem., 72, 248, 1976
- Adinarayana K, Ellaiah P, Srinivasulu B, Devi RB, Adinarayana G, Process Biochem., 38(11), 1565, 2003
- Esan TA, Sobukola OP, Sanni LO, Bakare HA, Munoz L, Food Bioprod. Process., 95, 27, 2015
- Kenari SLD, Alemzadeh I, Maghsodi V, Food Bioprod. Process., 89, 315, 2011
- Prakasham RS, Rao CS, Rao RS, Rajesham S, Sarma PN, Appl. Biochem. Biotechnol., 120, 133, 2004
- Rao RS, Prakasham RS, Prasad KK, Rajesham S, Sarma PN, Rao LV, Process Biochem., 39(8), 951, 2004
- Srinivasulu B, Prakasham RS, Jetty A, Srinivas S, Ellaiah P, Ramakrishna SV, Process Biochem., 38(4), 593, 2002
- Glick BR, Biotechnol. Adv., 13, 247, 1995
- Bentley WE, Mirjalili N, Andersen DC, Davis RH, Kompala DS, Biotechnol. Bioeng., 35, 668, 1990
- Lecina M, Sarro E, Casablancas A, Godia F, Cairo JJ, Biochem. Eng. J., 70, 78, 2013
- Agarwal A, Kumar S, Veeranki VD, Lett. Appl. Microbiol., 52, 307, 2011
- Jia M, Xu M, He B, Rao Z, J. Agric. Food Chem., 61, 9428, 2013
- Jacques P, Hbid C, Destain J, Razafindralambo H, Paquot M, De Pauw E, Thonart P, Appl. Biochem. Biotechnol., 77, 223, 1999
- Commichau FM, Forchhammer K, Stulke J, Curr. Opin. Microbiol., 9, 167, 2006
- Hymavathi M, Sathish T, Brahmaiah P, Prakasham RS, Chem. Biochem. Eng. Q., 24, 473, 2010
- Das K, Mukherjee AK, Process Biochem., 42(8), 1191, 2007
- Dauner M, Storni T, Sauer U, J. Bacteriol., 183, 7308, 2001
- Tian Y, Fan Y, Zhao X, Zhang J, Yang L, Liu J, Prep. Biochem. Biotechnol., 44, 529, 2014
- Dutta K, Dasu VV, Hegde K, Adv. Microb. Physiol., 3, 479, 2013
- Kim M, Si JB, Reddy LV, Wee YJ, RSC Adv., 6, 51270, 2016
- da Costa HM, Ramos VD, da Silva WS, Sirqueira AS, Polym. Test, 29, 582, 2010
- Draper NR, Applied Regression Analysis, Wiley (1981).
- Prabhu AA, Chityala S, Garg Y, Dasu VV, Prep. Biochem. Biotechnol., 47, 236, 2017
- Liu W, Zhao W, Lai J, Shen Q, Xu Y, Pan L, Chen S, Biotechnol. Appl. Biochem. (2016), DOI:10.1002/bab.1532.
- Sanjay K, Anand AP, Veeranki VD, Kannan P, Korean J. Chem. Eng., 34, 118, 2016
- Barnes WR, Dorn GL, Vela GR, Appl. Environ. Microbiol., 33, 257, 1977
- Baskar G, Renganathan S, Asia-Pac. J. Chem. Eng., 7, 212, 2012
- Dinarvand M, Rezaee M, Foroughi M, Braz. J. Microbiol., 48, 427, 2017
- Ghoshoon MB, Berenjian A, Hemmati S, Dabbagh F, Karimi Z, Negahdaripour M, Ghasemi Y, Int. J. Pept. Res. Ther., 21, 487, 2015
- Mahajan RV, Saran S, Kameswaran K, Kumar V, Saxena RK, Bioresour. Technol., 125, 11, 2012
- Singh Y, Srivastav SK, Indian J. Exp. Biol., 51, 322, 2013
- Feng Y, Liu S, Jiao Y, Gao H, Wang M, Du G, Chen J, Appl. Microbiol. Biotechnol., 101, 1509, 2016
- Fatiha B, Sameh B, Youcef S, Zeineddine D, Nacer R, Prep. Biochem. Biotechnol., 43, 33, 2013
- Muthuraj M, Chandra N, Palabhanvi B, Kumar V, Das D, BioEnergy Res., 8, 726, 2015
- Rahimpour F, Hatti-Kaul R, Mamo G, Process Biochem., 51(3), 452, 2016
- Singh P, Shera SS, Banik J, Banik RM, Bioresour. Technol., 137, 261, 2013
- Zafar M, Kumar S, Kumar S, Dhiman AK, Biocatal. Agric Biotechnol.
- Gurunathan B, Sahadevan R, Biotechnol. Bioprocess Eng., 16, 50, 2011
- Kumar S, Dasu VV, Pakshirajan K, Process Biochem., 45(2), 223, 2010
- Deshpande N, Choubey P, Agashe M, Scientific World J. (2014), DOI:10.1155/2014/895167.
- Windholz M, The Merck Index: An Encyclopaedia of Chemicals, Drugs and Biologicals, 10th Ed. NJ: Merck and Co. (1983).
- Caulfield MJ, Qiao GG, Solomon DH, Critical Reviews, 102, 3067, 2002
- Nandana V, Singh S, Singh AN, Dubey VK, Protein Expr. Purif., 103, 16, 2014
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