| Search / Korean Journal of Chemical Engineering |
Korean Chemical Engineering Research,
Vol.48, No.6, 690-694, 2010
Vol.48, No.6, 690-694, 2010
한방목초액의 항산화 및 항 아토피 효과
Anti-oxidation and Anti-atopic Dermatitis Effect of Herbal Wood Vinegar
본 연구에서는 대나무, 계피, 생강 등 8종의 한약재를 포함한 한방목초액의 소재특성을 시험하여 화장품 및 피부질 환용 의약품에의 응용가능성을 조사하였다. 목초액의 항산화력은 DPPH 자유라디칼 소거법으로 측정되었으며 50 μg/ml의 농축목초액 농도에서 97%의 매우 높은 항산화능을 보여주었다. 목초액의 항균력은 원판확산법으로 시험되었으며, 피부상재균인 Staphylococcus aureus에 대해 우수한 항균력을 보여주었다. 목초액의 미백효과는 tyrosinase 활성억제시 험법으로 측정되었으며 대조군인 비타민 C에 비해 매우 낮아서 미백효과는 미미하였다. 목초액의 안전성 시험은 MTT assay에 의해 측정되었으며, 세포 독성이 비교적 높은 것으로 나타났다. 목초액의 화장품소재 안정성 시험 결과 색, 향, 외관 및 pH에서 큰 변화가 없어서 매우 안정하였다. 무모생쥐를 이용한 항 아토피 시험결과, 목초액은 DNCB에 의해 유도된 피부염증을 9일 째 거의 정상상태로 회복시켰으며, 피부내 IgE의 농도도 대조군에 비해 30% 감소시켜 항 아토피 효과가 우수한 것으로 나타났다. 따라서 목초액은 항산화력, 항균력 및 항 아토피 효과가 우수하여 화장품소재나 피부질환치료제로서의 응용 가능성이 큼을 알 수 있었다.
In this study, herbal wood vinegar including Bambusoideae, Cinnamomi Cortex, Zingiberis Rhizoma was tested to see possibility for cosmetic or skin related medicine. Anti-oxidation effect of herbal wood vinegar was tested by DPPH free radical scavenging activity, and showed 97% inhibition rate at 50 μg/ml. Anti-bacterial effect was tested by disc diffusion method, and it indicated strong anti-bacterial activity against normal skin flora Staphylococcus aureus. Whitening effect was measured by tyrosinase inhibition assay, and it was lower compared with vitamin C. Stability test was done by MTT assay, and cell toxicity was relatively high. Stability was also checked, and there was not significant change in color, aroma, appearance and pH during storage. Anti-atopic dermatitis test was done by hairless mouse and herbal wood vinegar recovered damaged skin to almost normal condition after 9 days of application. IgE concentration
in herbal wood vinegar treated mouse was also reduced 30% compared with control. From the study, herbal wood vinegar showed good anti-oxidation, anti-bacterial and anti-atopic dermatitis effect, and had promising application in cosmetic or skin related medicine.
[References]
- KDA Textbook Editing Board, Dermatology, 5th ed., Ryo Mook Gak, Seoul, 2008
- Jung IS, Kim YJ, Gal SW, Choi YJ, J. Life Science, 17, 105, 2007
- Lee KM, Jeong GT, Park DH, Korean J. Biotechnol. Bioeng., 19, 381, 2004
- Byun MW, “Atopic Dermatitis Improving Wood Vinegar,” Korea Patent No. 10-2007-0042868.
- Kim SW, Choi DH, Lee SM, Nam JJ, Kim HM, Shon SY, Song BH, Korean J. Organic Agriculture, 11, 103, 2003
- Park JC, Functional Food and Oriental Medicine, Hyoilbooks, Seoul, 2007
- Pharmacognosy Researchers, Modern pharmacognosy, Hak Chan Publishing, Seoul, 2000
- Park SK, Hong SK, Kim HJ, Kim BY, Kim T, Kang JS, Kim D, Korean Chem. Eng. Res., 47(5), 553, 2009
- Association of Official Analytical Chemists, A.O.A.C., Official Methods of Analysis, 15th ed., Washington DC., 1990
- Ishihara Y, Oka M, Tsunakawa M, Tomita K, Hatori M, Yamamoto H, Kamei H, Miyaki T, Konishi M, Oki T, J. Antibiotics, 44, 25, 1991
- Korea Food and Drug Administration, http://www.kfda.go.kr/index.html.
- Mosmann T, J. Immun Method, 65, 55, 1983
- Kang KJ, “Anti-Atopic Dermatitis Effect of Prunus Yedoensis Matsum Bark Extract,” Master’s thesis, College of Medicine, Jeju University, 2006
- Kim JY, Yang HJ, Lee KH, Jeon SM, Ahn YJ, Won BR, Park SN, J. Soc. Cosmet. Scientists Korea, 33, 165, 2007
- Kim DH, Choi HK, Cho SC, Kook MC, Park CS, J. Soc. Cosmet. Scientists Korea, 34, 225, 2008
참고문헌정보(참고문헌, 인용문헌)는 화학공학소재연구정보센터에 의해 제공되고 있습니다.