Porous TiO2 nanostructured particles containing both mesopores and macropores were fabricated by utilizing an aerosol templating method from two kinds of starting materials (colloidal mixture of TiO2 nanoparticles and PS particles, and that of TTIP solution and PS particles). The effects of mixing ratio of PS to TiO2 and reactor temperature on the particle properties were investigated. When TiO2 nanoparticles were used as starting materials, the increase of macropores number was observed by SEM and the specific surface area and total pore volume were increased from 31.6m2/g to 39.1 m2/g and 0.068 cm3/g to 0.089 cm3/g, respectively, by increasing the weight mixing ratio of PS/TiO2 from 0.79 to 1.31. When TTIP was used as precursor, the specific surface area and mesopore volume of particles prepared at same condition decreased by 67% and 75%, respectively.