|
Search / Korean Journal of Chemical Engineering
|
Korean Chemical Engineering Research, Vol.42, No.6, 669-672, 2004
아임계/초임계 수에 의한 토종콩으로부터 이소플라본의 추출
Extraction of Isoflavones from Korean Soybean by Sub/Supercritical Water
토종콩으로부터 비배당체 화합물을 아임계/초임계 수(水)를 이용하여 추출하였다. 이 때, 실험 조건은 아임계 및 초임계 수의 온도와 압력으로 하였다. 추출된 용액은 역상 고성능 액체 크로마토그래피(RP-HPLC)를 사용하여 분석 하였다. 200 ℃, 200 bar의 아임계 상태의 실험 조건에서 비배당체들이 선택적으로 추출되었다. 또한 400 ℃, 250 bar의 초임계 상태에서 5 g의 토종콩으로부터 추출된 비배당체들의 양(0.9 μg)과 비슷한 것을 실험을 통해 확인하였다.
Aglycones compounds were extracted from Korean soybean by sub/supercritical water. The various experimental variables were the temperature and pressure of sub/supercritical water. The extracted sample was analyzed by reversed-phase high performance liquid chromatography (RP-HPLC). Aglycons were selectively extracted at the experimental condition of 200 ℃ and 200 bar of subcritical water. It was also experimentally observed that almost the same amount of aglycones, 0.9 μg, was extracted from 5 g of Korean soybean at 400 ℃ and 250 bar of supercritical water.
[References]
- Barnes S, Kirk M, Coward L, J. Agric. Food Chem., 42, 2466, 1994
- Xia X, Huei-Ju W, Murphy PA, Cook L, Hendrich S, J. Nutr., 124, 825, 1994
- Kim CH, Park JS, Sohn HS, Chun CW, Korean J. Food Sci. Technol., 34(1), 96, 2002
- Kim JS, Yoon S, Korean J. Food Sci. Technol., 31(6), 1405, 1999
- Lee KJ, Choi DY, Row KH, HWAHAK KONGHAK, 41(5), 612, 2003
- Boo SJ, Byun SY, Korean J. Biotechnol. Bioeng., 16(1), 95, 2001
- Bahram H, Arjmandi B, Smith J, J. Nutr. Biochem., 13, 130, 2002
- Vendula K, Jana P, Michael B, Theriogenology, 61, 1307, 2004
- Song MS, Lee YW, Kim JD, Row KH, HWAHAK KONGHAK, 41(2), 207, 2003
- Maurcio A, Rostagno MA, Julio A, Sandi D, Food Chem., 78, 111, 2002
- Bezman RD, Casassa EF, Kay RL, J. Mol. Liq., 74, 397, 1997
- Ra YJ, Lee YW, Kim JD, Row KH, Korean J. Biotechnol. Bioeng., 16(4), 327, 2001
- Peter K, Eckhard D, J. Chem. Eng., 83, 207, 2001
- Wagner W, Pruβ A, J. Phys. Chem. Ref Data, 31, 387, 2002
- Sears FW, Zemansky MW, Young HD, University Physics, 6th Ed., Addison-Wesley, 1982
- Chien JT, Hsieh HC, Chen BH, Kinetic Model for Studying the Conversion and Degradation of Isoflavones During Heating," Food Chemisty(in press).
[Cited By]
- Lee KJ, Jin LM, Kim YS, Row KH, Korean Chemical Engineering Research, 43(5), 641, 2005
- Wan X, Jung YA, Row KH, Korean Chemical Engineering Research, 46(4), 660, 2008
|