Search / Korean Journal of Chemical Engineering
Korean Chemical Engineering Research,
Vol.42, No.6, 669-672, 2004
아임계/초임계 수에 의한 토종콩으로부터 이소플라본의 추출
Extraction of Isoflavones from Korean Soybean by Sub/Supercritical Water
토종콩으로부터 비배당체 화합물을 아임계/초임계 수(水)를 이용하여 추출하였다. 이 때, 실험 조건은 아임계 및 초임계 수의 온도와 압력으로 하였다. 추출된 용액은 역상 고성능 액체 크로마토그래피(RP-HPLC)를 사용하여 분석 하였다. 200 ℃, 200 bar의 아임계 상태의 실험 조건에서 비배당체들이 선택적으로 추출되었다. 또한 400 ℃, 250 bar의 초임계 상태에서 5 g의 토종콩으로부터 추출된 비배당체들의 양(0.9 μg)과 비슷한 것을 실험을 통해 확인하였다.
Aglycones compounds were extracted from Korean soybean by sub/supercritical water. The various experimental variables were the temperature and pressure of sub/supercritical water. The extracted sample was analyzed by reversed-phase high performance liquid chromatography (RP-HPLC). Aglycons were selectively extracted at the experimental condition of 200 ℃ and 200 bar of subcritical water. It was also experimentally observed that almost the same amount of aglycones, 0.9 μg, was extracted from 5 g of Korean soybean at 400 ℃ and 250 bar of supercritical water.
[References]
  1. Barnes S, Kirk M, Coward L, J. Agric. Food Chem., 42, 2466, 1994
  2. Xia X, Huei-Ju W, Murphy PA, Cook L, Hendrich S, J. Nutr., 124, 825, 1994
  3. Kim CH, Park JS, Sohn HS, Chun CW, Korean J. Food Sci. Technol., 34(1), 96, 2002
  4. Kim JS, Yoon S, Korean J. Food Sci. Technol., 31(6), 1405, 1999
  5. Lee KJ, Choi DY, Row KH, HWAHAK KONGHAK, 41(5), 612, 2003
  6. Boo SJ, Byun SY, Korean J. Biotechnol. Bioeng., 16(1), 95, 2001
  7. Bahram H, Arjmandi B, Smith J, J. Nutr. Biochem., 13, 130, 2002
  8. Vendula K, Jana P, Michael B, Theriogenology, 61, 1307, 2004
  9. Song MS, Lee YW, Kim JD, Row KH, HWAHAK KONGHAK, 41(2), 207, 2003
  10. Maurcio A, Rostagno MA, Julio A, Sandi D, Food Chem., 78, 111, 2002
  11. Bezman RD, Casassa EF, Kay RL, J. Mol. Liq., 74, 397, 1997
  12. Ra YJ, Lee YW, Kim JD, Row KH, Korean J. Biotechnol. Bioeng., 16(4), 327, 2001
  13. Peter K, Eckhard D, J. Chem. Eng., 83, 207, 2001
  14. Wagner W, Pruβ A, J. Phys. Chem. Ref Data, 31, 387, 2002
  15. Sears FW, Zemansky MW, Young HD, University Physics, 6th Ed., Addison-Wesley, 1982
  16. Chien JT, Hsieh HC, Chen BH, Kinetic Model for Studying the Conversion and Degradation of Isoflavones During Heating," Food Chemisty(in press).