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HWAHAK KONGHAK,
Vol.38, No.4, 474-478, 2000
Vol.38, No.4, 474-478, 2000
흡착평형식의 결정을 위한 Frontal Analysis와 Pulsed Input Method와의 비교
Comparison of Frontal Analysis and Pulsed Input Method for Determining Adsorption Isotherm
크로마토그래피 공정에서 가장 기본이 되는 것이 흡착평형식이다. 역상 액체크로마토그래피에서 thymidine과 5'-GMP의 흡착평형을 구하기 위해 frontal analysis(FA)와 pulsed-input method(PIM)의 두 가지 방법을 이용하였다. Langmuir 흡착평형식으로 가정하여 매개변수 a와 b값을 FA와 PIM의 방법에 의해서 구하였다. FA에서는 고정상의 농도를 용출곡선으로부터 측정하고 회귀분석을 하였고 PIM에서는 Langmuir 흡착평형식의 매개변수를 최적화방벙에 의해 구하였다. FA와 PIM의 방법으로부터 구한 thymidine과 5'-GMP의 흡착평형식은 유사하였다. FA 방법에 비해서 PIM의 장점은 시료의 양이 적게 소모되고 실험방법이 간편하였다.
Adsorption isotherm is the most fundamental imformation related to chromatography process. To calculate the parameters of Langmuir adsorption isotherm, a and b, of thymidine and 5''''-GMP, frontal analysis(FA)and pulsed-inpot method (PIM)were adopted in RP-HPLC. In FA concentrations of stationary phase were measured from the elution curves and the isotherm was determined by regression analysis, while the parameters of the isotherm in PIM was obtained by optimization method. Comparing between results from methods, the adsorption isotherms of thymidine and 5''''-GMP were very similar. The superity of PIM over FA were that the consumption of sample was less and only one or two injections of sample were required.
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