Search / Korean Journal of Chemical Engineering
HWAHAK KONGHAK,
Vol.38, No.1, 26-31, 2000
Stirred Cell 안에서 BSA의 흡착 과정에 의해 응집된 Microsphere가 투과 Flux에 미치는 영향 고찰
Study on the Permeate Flux in the Filtration of BSA-Adsorbed Microsphere Using Stirred Cell
본 논문은 stirred cell 안에서 BSA가 microsphere에 흡착되는 과정중의 투과 flux의 변화에 관한 연구 결과이다. 모델 단백질로 널리 사용되는 BSA 용액의 농도(0.1, 0.2, 0.4 g/L)와 stirrer speed(300, 400, 600 rpm)를 변수로 하여 실험하였으며, 시간에 따른 투과 flux의 변화를 고찰하였다. 단백질을 투입한 후 시간이 지남에 따라 투과 flux는 증가하다가 다시 감소하는 경향을 보임을 알 수 있었다. Hydrophobic interaction에 의해 BSA가 microsphere에 흡착되며 이 BSA-absorbed microsphere는 흡착이 계속 진행되면서 유효 입자 크기가 커지게 된다. 이러한 이유로 투과 flux는 증가하게 된다. 입자의 크기가 크면 투과 flux가 커진다는 사실은 잘 알려져 있다. BSA에 의해 포화 흡착된 후에는 더 이상 흡착이 진행되지 못하고 BSA는 stirred cell에 장착된 막을 통과하여 빠져 나오게 된다. 이때 BSA가 막 기공 안쪽에 흡착되어 투과 flux는 감소하게 된다. BSA 용액의 농도가 높아지게 되면 포화 흡착이 빨리 일어나기 때문에 투과 flux가 증가하는 부분이 짧아짐을 알 수 있었으며, stirrer speed가 높아질수록 투과 flux는 증가하였다.
A study on permeate flux variation in the filtration of BSA-adsorbed microspheres was performed, to investigate the effects of the stirrer speed (300, 400 and 600 rpm) and the concentration of BSA solutions (0.1, 0.2 and 0.4 g/L). The permeate flux was increased with time until the break point, but decreased after that point. This is in contrast to the observation from the conventional filtration. It could be explained that the mircosphers which were adsorbed by BSA agglutinated each other, and resulted in the increase of effective particle diameter of BSA-adsorbed microsphere. It is well known that the large-size substances make flux increase. When BSA''s were saturated on the surface of microspheres, they permeated through membrane and some of them absorbed inside the pore of membrane, resulting in the flux decline as observed in the general filtration. The period of flux increase was shortened with an increase in the concentration of BSA solution and permeate flux was increased with a increase in the stirrer speed.
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