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HWAHAK KONGHAK,
Vol.41, No.3, 301-306, 2003
Vol.41, No.3, 301-306, 2003
단이론에 의한 주입부피와 농도의 변화에 따른 용출곡선의 예측
Estimation of Elution Profiles with Injection Volumes and Concentrations by Plate Theory
크로마토그래피 분리에서 주입부피와 농도에 따라서 피크 폭이 변하게 된다. 단이론을 통해 가우시안 형태를 갖는 피크의 용출곡선을 예측하였다. 선형적인 흡착평형식으로 표시되는 2-deoxycytidine(dCyd)에 대해서 주입부피와 농도에 따른 실험값과 계산된 용출곡선을 비교하였다. 주입부피가 커지는 경우에서는 정확한 HETP를 계산하기 위해서 Hinjection을 고려해야 한다. 또한 비선형 흡착평형식을 갖는 2-deoxyadenosine(dAdo)에 대해서도 용출곡선을 속도론에 의하여 계산하였다. 주입부피와 농도에 따라서 체류시간이 유사한 dCyd의 가우시안 피크는 단이론에 의해서 예측이 가능하였지만 dAdo는 Langmuir 비선형 흡착식으로 나타냈고 속도론에 의한 계산 결과는 실험값과 일치하였다.
In a chromatographic separation, the width of a peak was affected by injection volume and concentration. The elution profiles of Gaussian peaks were predicted by the plate theory. For 2-deoxycytidine(dCyd), the experimental data with injection volumes and concentrations were compared with the calculated elution profiles in linear equilibrium. When the injection volume was larger, Hinjection should be included to calculate HETP accurately. Also for 2-deoxyadenosine(dAdo), elution profiles were calculated by the rate theory in nonlinear isotherm. The good agreements between the two values of dCyd by plate theory and of dAdo by rate theory were observed.
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