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Search / Korean Journal of Chemical Engineering
HWAHAK KONGHAK,
Vol.32, No.4, 622-626, 1994
Amyloglucosidase에 의한 Fructo-oligosaccharides 생산의 반응메카니즘
Reaction Mechanism for the Production of Fructooligosaccharides by Amyloglucosidase
윤종원, 이민규, 송승구
Amyloglucosidase에 의한 fructooligosaccharides 생산에서의 효소반응 메카니즘을 수학적 모델을 통해 규명 하였다. Amyloglucosidase는 기존의 fructooligosaccharides 생산효소인 fructosyltransferase와 거의 동일한 메카니즘에 의한 fructooligosaccharides를 생산하는 것으로 밝혀졌다. 효소 동력학적 인자 중 Km 및 KI 값들은 fructosyltransferase에 비해 큰 값을, Vmax 는 낮은 값을 나타내었다. 제안된 모델을 두 기질농도에 대한 실험치와 비교해 본 결과 대체로 서로 잘 일치하였다.
A mathematical model for the production of fructooligosaccharides by amyloglucosidase was proposed and compared with experimental results. The reaction mechanism of amyloglucosidase in fructooligosaccharides production was very similar to that of fructosyltransferase except that fructose was accumulated at a high level. The kinetic parameters such as Michaelis constants(Km) and inhibition constant(KI) were higher, whereas maximum reaction velocities(Vmax) were low compared with those of fructosyltransferase. Although data points were scattered for profiles of sucrose and 1-kestose to some extent, good agreement was generally found between the proposed model and experimental results.
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