Mixed organic solvent was used as a medium instead of buffer solution in the new developed filter paper electrophoresis cell. The sample protein solution was prepared as the micro-aqueous system and the protein of the sample protein solution migrated at the high electrical voltage of 7 kilo volts applied between two electrodes. We could select usable protein organic solvents by measuring the electrical conductivities of organic solvents and checking the stability of the sample protein solution. The protein of the sample protein solution moved lump by lump. The direction of the protein movement was reverse and the migration speed was more than 100 times faster than that of conventional electrophoresis system.