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Search / Korean Journal of Chemical Engineering
HWAHAK KONGHAK,
Vol.40, No.1, 100-105, 2002
전기장 인가에 따른 DNA 신장에 관한 AFM 연구
An AFM Study for Stretching Electric Field Induced DNA
박진영, 한성웅, 손세영, 송성훈, 김종민, Ohtani T, Muramatsu H, 장상목
본 연구에서는 마이카 표면상에서 전기장에 의한 극히 낮은 농도의 DNA 신장에 대한 효율적인 방법론을 제시하였다. 전기장에 의한 DNA 신장을 AFM형상으로 분석한 결과, DNA 농도범위가 약 57×10(-3) ng/mL-57×10(-6) ng/mL 일 때 매우 잘 적용되며, 신장된 DNA는 두 전극 중앙에 존재하였다. 또한, 일반 마이카, Mg(2+) 처리된 마이카, 그리고 AP-마이카 표면들에 대한 신장 효율의 차를 기술하였다. 동일한 실험조건 하에서 AP-마이카의 표면이 가장 좋은 효율의 DNA 신장 결과를 나타내었다.
An effective method of DNA stretching using electric field on mica surface was proposed for extremely low solution concentration of DNA. DNA stretching based on electric field was observed by AFM and well applied on the concentration range from 57×10(-3) ng/mL to 57× 10(-6) ng/mL. Stretched DNA existed in the middle of two electrodes. The difference in stretching efficiency for the different surfaces of bare mica, Mg(2+) soaked mica and AP-mica was discussed. The best performance of stretching was found from the surface of AP-mica under the same experimental condition.
Keywords: AFM; NSOM; DNA; Mica
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[Cited By]
  1. Park YG, Lim HE, Journal of Industrial and Engineering Chemistry, 8(5), 419, 2002
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