| Search / Korean Journal of Chemical Engineering |
HWAHAK KONGHAK,
Vol.31, No.6, 846-851, 1993
Vol.31, No.6, 846-851, 1993
Fructosyltransferase와 Glucose Isomerase 혼합효소계를 이용한 프락토 올리고당의 생산
Production of Fructo-oligosaccharides by the Mixed-enzyme System of Fructosyltransferase and Glucose Isomerase
프락토 올리고당 생산 효소인 fructosyltransferase와 glucose isomerase와의 혼합효소계를 이용하여 새로운 조성의 프락토 올리고당 생산을 검토하였다. Fructosyltransferase의 효소화학적 특성인 glucose 저해현상을 glu-cose isomerase에 의해 낮추어 주었음에도 불구하고 프락토 올리고당의 전화률을 크게 증가시킬 수 없었다. 이 현상을 설명하기 위한 수학적 모델을 제안하고 실험을 통해 확인한 결과, fructosyltransferase 단일효소계와 비교할 때 혼합효소계의 경우 여러 가지 기질에 대한 동력학적 상수(Km, Ki)의 값이 현저하게 달라짐을 알 수 있었다. Glucose isomerase에 의해 생산된 fructose 는 fructosyltransferase에 의해 전이되지 않아 프락토 올리고당의 농도를 증가시켜 주지 못하는 것으로 보아 프락토 올리고당의 생산반응을 fructosyltransferase의 selftrans-fer 반응에 의해서만 생산된다는 결론을 얻었다.
The production of fructo-oligosaccharides having new composition was investigated using the mixed-enzyme system of fructosyltransferase and glucose isomerase. Despite of decrease in glucose concentration which acts as an inhibitor of fructosyltransferase during the production of fructo-oligosaccharides, the concentration of fructo-oligosaccharides was not high due to the altered kinetic parameters in the mixed-enzyme system was proposed and compared with the experimental results, which showed good agreement with experimental data. Surpris-ingly, the Km values and inhibition constant of glucose(KiG) for fructosyltransferase in the mixed-enzyme system were different from those of fructosyltransferase alone. The fructose isomerized from glucose by glucose isomerase did not act as a sucrose acceptor, which meant the isomerized fructose did not play a part in the production of fructo-oligosaccharides.
[References]
- 편집부: Food Chemicals(Japan), Oct., 10, 1989
- 편집부: 식품과 개발(일본), 26(10), 23, 1990
- Yun JW, Jung KH, Oh JW, Lee JH, Appl. Biochem. Biotechnol., 24-25, 299, 1990
- Hidaka H, Hirayama M, SumiM, Agric. Biol. Chem., 52, 1181, 1988
- Hayashi S, Nonokuchi M, Imada K, Ueno H, J. Ind. Microbiol., 5, 395, 1990
- Kohomoto T, Tsuji K, Kaneko T, Shiota M, Fukui F, Takaku H, Nakagawa Y, Ichikawa T, Kabayashi S, Biosci. Biotech. Biochem., 56(6), 937, 1992
- Jung KH, Lim JY, Yoo SJ, Lee JH, Yoo MY, Biotechnol. Lett., 9, 703, 1987
- Yun JW, Jung KH, Jeon YJ, Lee JH, J. Microbiol. Biotechnol., 2(2), 98, 1992
- vanBalken JAM, vanDooren JGM, vandenTweel WJJ, Kamphuis J, Meijer EM, Appl. Microbiol. Biotechnol., 35, 216, 1991
- Jung KH, Yun JW, Kang KR, Lim JY, Lee JH, Enzyme Microb. Technol., 11, 491, 1989
- Yun JW, Jeon YJ, Lee MG, Song SK, 한국생물공학회지, 8(3), 266, 1993
- Yun JW, Song SK, Biotechnol. Lett., 15(6), 573, 1993
- Gupta AK, Bhatia IS, Phytochemistry, 19, 2557, 1980
- Takasaki Y, Agric. Biol. Chem., 31(3), 309, 1967
- Hemmingsen SH, Applied Biotechnology and Bioengineering Vol. 2, p. 157, Academic Press Inc., New York, 1979
- Fujita K, Hara K, Hashimoto H, Kitahata S, Agric. Biol. Chem., 54(10), 2655, 1990
[Cited By]
참고문헌정보(참고문헌, 인용문헌)는 화학공학소재연구정보센터에 의해 제공되고 있습니다.