Vindoline and catharanthine were extracted from the pretreated leaves of Catharanthus roseus using supercritical carbon dioxide and their mass transfer coefficients were determined in various conditions of temperature and pressure. Extraction yields of vindoline and catharanthine were compared with the reduced density of supercritical carbon dioxide and the relational parameters were calculated in each given temperature. Defining the mass transfer coefficient of vindoline and catharanthine between solid phase and fluid phase, the mass transfer was studied by steady state analysis. Sherwood number obtained by this method was expressed as a function of Reynolds and Schmidt number.