The enzymatic degradation of polycaprolactone(PCL) was investigated using lipases obtained from different microorganisms by measurement of weight loss and total organic carbon(TOC) concentration. Lipase from Pseudomonas sp. was able to degrade PCL most efficiently. In this study, three major variable, enzyme concentration, substrate concentration, and reaction time, were optimized to enhance biodegradation of PCL. The optimal condition of diodegradation of PCL was achieved at the substrate concentration of 30g/L, the enzyme concentration of 30mg/L, and the degradation time of 15-20hours. Three different forms of PCL, film, fine powder, and coarse powder, were investigated to understand the effect of surface area on the degradation rate. PCL with large surface area(fine powder) was degraded most efficiently by lipase obtained from Pseudomonas sp.