This study examines the potential of dahlia pinnata as an alternative energy source for ethanol. Experimental results are presented as batch fermentation kinetics for two strains of Kluyveromyces fragilis CBS 1555 and Saccharomyces cerevisiae ATCC 4126 grown on the extract derived from the tubers of Dahlia pinnata. In the first extraction stage, the range of sugar conentration was 90-203g/l in terms of total sugars. Saccharomyces cerevisiae cells were immobilized in Ca-alginate beads and used in a packed-bed bioreactor for the continuous production of ethanol from the extract of dahlia pinnata tubers. Ethanol yield varied from 0.92g-EtOH/g-Sugar utilized at D=0.1hr^-1 to 0.57g-EtOH/g-Sugar utilized at D=0.4hr^-1. The results presen-ted in this study show that immobilized cells of S. cerevisiae have a high potential for fuel ethanol production from Dahlia pinnata tubers. the immobilized cell bioreactor was operated continuously at a constant dilution rate of 0.18hr^-1 for 35 days. The maximun ethanol yield was found to be 0.98g-EtOH/g-Sugar.