Saccharomyces formosensis cells were immobilized in calcium alginate beads and used for a con-tinuous production of ethanol. The immobillized yeast cells were examined in a packed-bed reactor operated under various temperature and pH. Ethanol production rate with the immobilized yeast showed a temperature optimum of 33℃ and a broad range of optimum pH. Under the optimum conditions, ethanol productivity of 69.1-76.1g EtOH/lㆍh could be obtained by using 12.4%(W/V)glucose as the substrate.