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HWAHAK KONGHAK,
Vol.24, No.5, 407-413, 1986
Vol.24, No.5, 407-413, 1986
혼합효소에 의한 셀룰로오즈의 가수분해
Hydrolysis of Cellulose by Enzyme Mixture
Trichoderma viride cellulase의 약한 β-glucosidase의 활성을 보충해 주기 위해 Trichoderma viride cellulase와 Aspergillus niger cellulase를 혼합하여 여러 종류의 셀룰로오즈를 가수분해하였다.
혼합효소는 완전한 cellulase로 알려진 Trichoderma viride cellulase보다 효과적으로 셀룰로오즈를 분해하였다.
미세결정성 셀룰로오즈와 결정성 셀룰로오즈를 혼합효소로 48시간 가수분해하였을 때 당화율이 각각 53 및 85 %로 Trichoderma viride cellulase에 의한 당화율보다 1.32 및 1.15배 증가하였다.
혼합효소는 완전한 cellulase로 알려진 Trichoderma viride cellulase보다 효과적으로 셀룰로오즈를 분해하였다.
미세결정성 셀룰로오즈와 결정성 셀룰로오즈를 혼합효소로 48시간 가수분해하였을 때 당화율이 각각 53 및 85 %로 Trichoderma viride cellulase에 의한 당화율보다 1.32 및 1.15배 증가하였다.
To enhance the activity of β-glucosidase in enzyme system from Trichoderma viride, the cellulase from Trichoderma viride and Aspergillus niger were mixed and various celluloses were hydrolyzed by using the mixed cellulase.
The mixed enzyme had an effect on hydrolysis of the cellulosic materials more than cellulase from Trichoderma viride. The saccharification percentages of microcrystalline and noncrystalline cellulose after 48 hr hydrolysis were 53 and 85 % respectively.
These saccharification values were 1.32 and 1.15 times as high as those of cellulase from Trichoderma viride.
The mixed enzyme had an effect on hydrolysis of the cellulosic materials more than cellulase from Trichoderma viride. The saccharification percentages of microcrystalline and noncrystalline cellulose after 48 hr hydrolysis were 53 and 85 % respectively.
These saccharification values were 1.32 and 1.15 times as high as those of cellulase from Trichoderma viride.
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