This paper deals with the enzymatic hydrolysis of different kinds of plant celluloses. The enzymatic hydrolysis of these materials by a complete cellulase from Trichoderma viride were carried out in the 0.15 M acetate buffer solution at pH 5.0, 50 ℃, and hydrolysis conditions were 25 mg cellulase, from Trichoderma viride for 100 mg plant cellulose and 72 hours.
The maximum yield of glucose from these experiments was 37.4 % in per cent saccharification for the indian corn stem without pretreatment for 72 hours. This enzymatic hydrolyzed solution of indian corn stem was concentrated by means of reversed osmosis until 100 mg/ml glucose concentrations. And this glucose solution was fermented into ethanol, using the strain Saccharomyces carlsbergensis w34, giving yields of 95-98 % of the theoretical value.