Langmuir-Blodgett hybrid films (CoSt2) were prepared with cobalt ion and stearic acid. We proposed a new post-treatment process which is easier and more efficient than the commonly used mehtods such as plasma treatment or H2S gas treatment. LB films were prepared depending on concentrations, pHs of subphase solution and the dipping number. In this study, UV treatment in the presence of H2O molecules sequencing skeletonization was proposed as a post-treatment technique; LB film is irradiated by UV lamp with water for 12 hours, followed by immersion of LB film into benzene stirred for 30 minutes. Analysis results of morphology for either skeletonization only or UV treatment showed that relatively large size particles (~50 nm) were formed, while UV treatment with water sequencing skeletonization showed that particle size formed on the substrate decreased up to 10 nm and cobalt molecules had regular electron pattern.