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HWAHAK KONGHAK,
Vol.21, No.5, 269-280, 1983
Vol.21, No.5, 269-280, 1983
미생물처리에 의한 볏짚으로부터 Furfural 제조에 관한 연구
Production of Furfural from Rice Straw by Microbial Treatment(I)-Production of xylose using immobilized enzymatic hydrolysis-
미생물을 이용하여 볏짚으로부터 Furfural을 제조하는데 있어서 제1단계로 Xylanase의 고정화와 그 효소의 반응계특성 및 고정층반응기에서 볏짚으로부터 추출된 Xylan의 연속적인 가수분해 반응에 관하여 연구하였다.
Xylalnase를 고정화시킬 때의 최적 plt와 온도는 pH5 및 20 ℃였으며, 고정화된 효소의 반응최적 pH와 온도는 각각 pH5 및 40 ℃였다.
또한 pH안정성 및 열안정성도 수용성 효소에 비하여 높게 나타났다.
활성탄에 고정화시킨 Xylanase를 사용하여 고정층반응기에서 연속적인 가수분해반응을 진행할 때, 볏짚으로부터 추출된 0.25∼1.25 % Xylan 용액의 8.2∼8.7 %가 Xylose로 전환되었으며, 기질의 유속이 증가하면 물질전달계수 kLα값의 증가로 겉보기 Km값이 감소하였으며, 이 계에서의 Km값은 0.82×10-3M이었다.
Xylalnase를 고정화시킬 때의 최적 plt와 온도는 pH5 및 20 ℃였으며, 고정화된 효소의 반응최적 pH와 온도는 각각 pH5 및 40 ℃였다.
또한 pH안정성 및 열안정성도 수용성 효소에 비하여 높게 나타났다.
활성탄에 고정화시킨 Xylanase를 사용하여 고정층반응기에서 연속적인 가수분해반응을 진행할 때, 볏짚으로부터 추출된 0.25∼1.25 % Xylan 용액의 8.2∼8.7 %가 Xylose로 전환되었으며, 기질의 유속이 증가하면 물질전달계수 kLα값의 증가로 겉보기 Km값이 감소하였으며, 이 계에서의 Km값은 0.82×10-3M이었다.
The study on immobilization of xylanase extracted from the culture of Aspergillus niger KG 79 was performed by attaching it covalently on activated carbon with glutaraldehyde as a bifunctional reagent. And the characteristics of reaction of immobilized enzyme and continuous hydrolytic reaction of xylan extracted from rice straw were studied.
Optimal values of pH and temperature for immobilization of xylanase were pH 5 and 20 ℃. In the hydrolytic reaction, the optimal pH and temperature were pH 5 and 40 ℃ respectively.
Xylanase in the immobilized state were more stable than those in the free state.
During the continuous hydrolytic reaction of xylan, 8.2∼8.7 % of xylan solution that has 0.25∼1.25 % concentration range, was converted to xylose and Km value of xylanase was 0.82×10-3M. As the flow rate of substrate solution was increased, apparent Km was reduced, because of the increasing mass transfer coefficient.
Optimal values of pH and temperature for immobilization of xylanase were pH 5 and 20 ℃. In the hydrolytic reaction, the optimal pH and temperature were pH 5 and 40 ℃ respectively.
Xylanase in the immobilized state were more stable than those in the free state.
During the continuous hydrolytic reaction of xylan, 8.2∼8.7 % of xylan solution that has 0.25∼1.25 % concentration range, was converted to xylose and Km value of xylanase was 0.82×10-3M. As the flow rate of substrate solution was increased, apparent Km was reduced, because of the increasing mass transfer coefficient.
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