|
Search / Korean Journal of Chemical Engineering
|
Korean Chemical Engineering Research, Vol.56, No.3, 370-375, 2018
분별침전에서 친수성 고분자 물질을 이용한 (+)-dihydromyricetin의 입자크기 감소
Decreasing Particle Size of (+)-Dihydromyricetin Using Hydrophilic Polymer in Fractional Precipitation
본 연구에서는 생리활성물질 (+)-dihydromyricetin의 입자크기 감소를 위하여 친수성 고분자 물질을 첨가하여 분별 침전을 수행하였다. 고분자 물질(HPMC 2910, PVP K90, PVA)이 첨가된 분별침전을 통해 (+)-dihydromyricetin 입자 크기를 감소시킬 수 있었다. 특히 분별침전 시 HPMC 2910 (0.1%, w/v)을 첨가할 경우, 대조군에 비하여 입자크기가 38-68 μm (32-40%) 정도 감소하여 입자크기 감소에 가장 효과적이었다. 또한 (+)-dihydromyricetin침전물의 입자크기는 친수성 고분자 물질 첨가에 따른 침전용액의 제타전위 절대값에 반비례함을 알 수 있었다.
In this study, we have applied fractional precipitation with hydrophilic polymer in order to decrease the particle size of the (+)-dihydromyricetin from plant materials. When compared with the case where no hydrophilic polymer was employed, the addition of hydrophilic polymer in fractional precipitation resulted in a considerable decrease in the size of the (+)-dihydromyricetin precipitate. Among the polymers used, HPMC 2910 was the most effective for inhibition of precipitate growth. A polymer concentration of 0.1% (w/v) yielded the smallest particle size. The particle size was reduced by ~40% compared to control. In addition, the precipitate size was inversely correlated with the absolute value of the zeta potential of the suspension with polymer.
[References]
- An SW, Kim YG, Kim MH, Lee BI, Lee SI, Lee SH, Kwon HI, Hwang B, Lee HY, Korean J. Medicinal Crop Sci., 7, 263, 1999
- Hase K, Basnet P, Kadota S, Namba T, J. Trad. Med., 14, 28, 1997
- Lee MK, Kim YG, An SW, Kim MH, Lee JH, Lee HY, Korean J. Medicinal Crop Sci., 7, 185, 1999
- Sakai K, Yamane T, Saitoh Y, Ikawa C, Nishihata T, Chem. Pharm. Bull., 35, 4597, 1987
- Yoo SM, Mun S, Kim JH, Process Biochem., 41(3), 567, 2006
- Du Q, Cai W, Xia M, Ito Y, J. Chromatogr. A, 973, 217, 2002
- Yohsikawa M, Murakami T, Chem Pharm Bull, 44, 1736, 1996
- Yohsikawa M, Murakami T, Ueda T, Yoshizumi S, Ninomiya K, Murakami N, Matsuda H, Satio M, Fujii W, Tanaka T, Yakugaku Zasshi., 117, 108, 1997
- Cho EB, Cho WK, Cha KH, Park JS, Int. J. Pharm., 396, 91, 2010
- Yeo SD, Kim MS, Lee JC, J. Supercrit. Fluids, 25(2), 143, 2003
- Pyo SH, Kim MS, Cho JS, Song BK, Han BH, Choi HJ, J. Chem. Technol. Biotechnol., 79, 1162, 2005
- Ruala J, Eerikaine H, Kauppinen EI, Int. J. Pharm., 284, 13, 2004
- Chen X, Young TJ, Sarkari M, Williams III RO, Johnston KP, Int. J. Pharm., 242, 3, 2002
- Vehring R, Pharm. Res., 25, 999, 2008
- Weers JG, Tarara TE, Clark AR, Expert Opin. Drug Deliv., 4, 297, 2007
- Kawashima Y, York P, Adv. Drug Deliv. Rev., 60, 297, 2008
- Lee KH, Kim JH, Biotechnol. Bioproc. Eng., 13, 274, 2008
- Lim MK, Kim JH, Korean J. Biotechnol. Bioeng., 42, 25, 2014
- Ha GS, Kim JH, Korean Chem. Eng. Res., 53(6), 831, 2015
- Dong Y, Ng WK, Shen S, Kim S, Tan RB, Int. J. Pharm., 375, 84, 2009
- Zhang HX, Wang JX, Zhang ZB, Le Y, Shen ZG, Chen JF, Int. J. Pharm., 374, 106, 2009
- Dalvi SV, Dave RN, Ind. Eng. Chem. Res., 48(16), 7581, 2009
- Labouret AD, Thioune O, Fessi H, Devissaguet JP, Puisieux F, Drug Dev. Ind. Pharm., 21, 229, 1995
- Stainmesse S, Orecchioni AM, Nakache E, Puisieux F, Fessi H, Colloid Polym. Sci., 273, 505, 1995
- Thioune O, Fessi H, Devissaguet JP, Puisieux F, Int. J. Pharm., 146, 233, 1997
- Pouretedal HR, Int. Nano Lett., 4, 103, 2014
- Kim MJ, Kim JH, Korean Chem. Eng. Res., 54(2), 278, 2016
[Cited By]
- Oh SR, Kim JH, Korean Journal of Chemical Engineering, 38(3), 480, 2021
- Lee HG, Kim JH, Korean Chemical Engineering Research, 60(2), 237, 2022
|