In this study, we optimized a method of PEO-PPO-PEO block copolymer embedding, for solving non-specific protein and biomolecular adsorption and high hydrophobicic surface property, which is widely known as problems of poly (dimethylsiloxane) (PDMS) that has frequently been used in basic biological and its applied research. We assessed its surface modification by controlling the concentration of embedded block copolymer, water-soaking time, and recovery time as variables by contact angle measurements. In order to evaluate its antifouling ability, adsorption of FITC-BSA molecules was quantified. Furthermore, we generated oil-in-water (O/W) emulsion as a proof-of-concept experiment to confirm that the optimized surface modification works properly.