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Search / Korean Journal of Chemical Engineering
Korean Chemical Engineering Research,
Vol.54, No.1, 89-93, 2016
식물세포배양으로부터 파클리탁셀 및 이의 반합성 전구체 10-디아세틸파클리탁셀의 분리 양상
Separation Behavior of Paclitaxel and Its Semi-synthetic Precursor 10-Deacetylpaclitaxel from Plant Cell Cultures
이충기, 김진현
본 연구에서는 식물세포배양으로부터 항암물질 파클리탁셀 및 이의 반합성 전구체 10-디아세틸파클리탁셀의 분리양상을 조사하였다. 식물세포인 바이오매스를 이용한 분리/정제 공정인 용매를 이용한 바이오매스 추출, 액-액 추출, 흡착제 처리, 헥산 침전, 분별 침전을 순차적으로 수행한 결과, 흡착제 처리 공정에서 10-디아세틸파클리탁셀는 파클리탁셀로부터 가장 효과적으로 분리됨을 알 수 있었다. 파클리탁셀 및 10-디아세틸파클리탁셀 분리에 적합한 흡착제 종류, 건조시료/흡착제 비율, 흡착제 처리 온도는 각각 실로퓨트, 1:1.5(w/w), 20 oC 이었다. 최적의 흡착제 처리 조건에서 10-디아세틸파클리탁셀은 74.1% 분리/회수 가능하였다.
In this study, we investigated the separation behavior of the anticancer agent paclitaxel and its semi-synthetic precursor 10-deacetylpaclitaxel (10-DAP) from plant cell cultures. As a result of sequential separation/purification performed by biomass extraction with solvent, liquid-liquid extraction, adsorbent treatment, hexane precipitation, and fractional precipitation, the adsorbent treatment was found to be the most effective in separating and recovering 10-DAP from paclitaxel. The optimal adsorbent type, crude extract/adsorbent ratio, and adsorbent treatment temperature were sylopute, 1:1.5 (w/w), and 20 oC, respectively. The separation/recovery of 10-DAP from paclitaxel was 74.1% in adsorbent treatment process under optimal conditions.
Keywords: Paclitaxel; 10-Deacetylpaclitaxel; Separation; Adsorbent Treatment; Optimization
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[Cited By]
  1. Han JH, Ji SB, Kim YS, Lee SH, Park SH, Kim JH, Korean Chemical Engineering Research, 55(6), 807, 2017
  2. Yang JW, Kim JH, Korean Chemical Engineering Research, 57(2), 210, 2019
  3. Kim HS, Kim JH, Korean Chemical Engineering Research, 57(2), 219, 2019
  4. Park SH, Kim JH, Korean Chemical Engineering Research, 58(1), 113, 2020
  5. Kang DY, Kim JH, Korean Journal of Chemical Engineering, 38(11), 2286, 2021
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