Limonene is orange flavored natural material that is mainly contained in mandarine and lemon peels. Dlimonene was extracted from cold-storaged mandarine peel by using Soxhlet extractor at 120 oC for 2 hours with ethanol as solvent. Henry constants of d-limonene and impurity were calculated as HLim=8.55 and Himp=0.223 from the result of HPLC analysis. 4-bed SMB of limonene simulation with 0.46×25 cm columns was conducted by using Aspen chromatography program. Then effective condition for purity was found by changing m2 and m3 values in triangle diagram. The highest purity was 98.59% at m2=2.57, m2=9.55. For this case, feed, desorbent, extract, and raffinate flow rates were 1 mL/min, 1.19 mL/min, 0.857 mL/min and 1.34 mL/min, respectively. Scale-up simulation was also conducted by increasing column diameter from 0.46 cm to 1.6 cm for getting the same efficiency. The increased flow rates were 12 mL/min, 14 mL/min, 10 mL/min, and 16 mL/min for feed, desorbent, extract, and raffinate. It was possible to scale-up with maintaining same limonene purity because linear isotherms of limonene and impurity were assumed.