Biological treatment is promising alternative to conventional air pollution control method. Bioreactors for air pollution control have found most of their success in the treatment of dilute and high flow waste air streams containing volatile organic compounds and odor. The studies of mass transfer in biotrickling filters for air pollution control were of importance in order to control and optimize the purification process. The objectives of this study were to develop the experimental methodologies to evaluate the mass transfer coefficients of gas/liquid(trickling liquid), gas/solid(biomass) and liquid/solid in three phase biotrickling filtration. Also, this study characterized the influence factors on mass transfer such as dynamic holdup volume, gas/liquid flow rate ratio, biomass weight in reactor and recirculation rate of trickling medium for each phase of biotrickling filter.