To study the effects of fabrication methods on the performance and durability of polymer electrolyte membrane fuel cells (PEMFCs), membrane-electrode assemblies (MEAs) were fabricated using a Dr blade method, a spray method, screen print method and screen print + spray method. The performance of single cells assembled with the prepared MEAs were initially measured and compared. Electrode accelerated stress testing (AST) involving a potentiostatic stepwave with 10 s at 0.6 V followed by 30 s at 0.9 V was applied to test durability of MEAs. Before and after 6,000cycles of the AST, I-V curves, impedance spectra, cyclic voltammograms, linear sweep voltammetry (LSV) and transmission electron microscope (TEM) were measured. Under the operating conditions, the Dr Blde MEA exhibited the highest initial performance. After electrode accelerated stress testing, screen print + spray MEA showed lowest degradation rate.