Search / Korean Journal of Chemical Engineering
Korean Chemical Engineering Research,
Vol.51, No.1, 42-46, 2013
미역으로부터 후코산틴 추출 및 후코산틴 안정성
Extraction of Fucoxanthin from Undaria pinnatifida and Stability of Fucoxanthin
미역에서 후코산틴을 추출하는 공정과 후코산틴의 안정성에 대해 연구하였다. 본 연구에서는 후코산틴 추출물을 기능성 식품에 이용하기 위해 용매를 에탄올을 사용했다. 에탄올 농도가 80%일 때 후코산틴 추출 농도가 최고였다. 50 ℃까지 추출온도가 상승할 때 추출수율이 증가하였다. 원료를 5분간 물세척하여 후코산틴 중 염분농도를 약 94% 감소시켰다. 후코산틴이 70 ℃에서 1일 저장 동안 30% 감소해, 70 ℃ 이상의 온도에서 열에 약함을 나타냈다. 그리고 빛 안정성 실험결과 후코산틴은 빛에도 매우 약함을 보였다.
Extraction process of fucoxanthin from Undaria pinnatifida and stability of fucoxanthin was studied. In this study, to utilized extracted components as a functional food material, ethanol was used as a solvent. The maximum concentration of fucoxanthin was obtained when 80% ethanol solvent was used. The extraction yield of fucoxanthin increased as extraction temperature raised to 50 ℃. Water washing of raw material for 5 minutes reduced the salt content about 94%. From the experiment that fucoxanthin content reduced by 30% for 1 day storage at 70 ℃, it was demonstrated that fucoxanthin was thermal-unstable above 70 ℃. And experimental result of light- stability showed that fucoxanthin was very unstable with light.
[References]
  1. Nina G, Sergiu A, Kathi G, Anja B, Claudia B, Josef W, Biochimica et Biophysica Acta., 1797, 543, 2010
  2. Thomas V, Jan B, Wolfram W, Maria M, Claudia B, Biochimica et Biophysica Acta., 1787, 905, 2009
  3. Lavanya P, Bruno R, Anja B, Claudia B, Biochimica et Biophysica Acta., 1797, 1647, 2010
  4. Nina G, Julia H, Kathi G, Claudia B, Josef W, Chem. Phys., 373, 104, 2010
  5. Liu CL, Liang AL, Hu ML, Toxicol. Vitro., 25, 1314, 2011
  6. Heo SJ, Jeon YJ, J. Photochem. Photobiol. B-Biol., 95, 101, 2009
  7. Hosokawa M, Kudo M, Maeda H, Kohno H, Tanaka T, Miyashita K, Biochim. Biophys. Acta., 1675, 113, 2004
  8. Das SK, Hashimoto T, Shimizu K, Yoshida T, Sakai T, Sowa Y, Komoto A, Kanazawa K, Biochim. Biophys.Acta., 1762, 328, 2005
  9. You SG, Yang C, Lee HY, Lee BY, Food Chem., 119, 554, 2010
  10. Kim KN, Heo SJ, Kang SM, Ahn G, Jeon YJ, Toxicol. Vitro., 24(6), 1648, 2010
  11. Hosokawa M, Kudo M, Maeda H, Kohno H, Tanaka T, Miyashita K, Biochimica et Biophysica Acta., 1675, 113, 2004
  12. Satomi Y, Nishino H, Biochimica et Biophysica Acta., 1790, 260, 2009
  13. Nakazawa Y, Sashima T, Hosokawa M, Miyashita K, Journal of Functional Foods., 1, 88, 2009
  14. Swadesh KD, Hashimoto T, Kanazawa K, Biochimica et Biophysica Acta., 1780, 743, 2008
  15. Bae JS, Lee JS, Kim YS, Sim WJ, Lee H, Chun JY, Park KP, Korean Chem. Eng. Res., 46(5), 886, 2008
  16. Heo SJ, Yoon WJ, Kim KN, Ahn GN, Kang SM, Kang DH, Affan A, Oh C, Jung WK, Jeon YJ, Food Chem. Toxicol., 48, 2045, 2010
  17. Woo MN, Jeon SM, Kim HJ, Lee MK, Shin SK, Shin YC, Park YB, Choi MS, Chemico-Biological Interactions., 186, 316, 2010
  18. Hosokawa M, Miyashita T, Nishikawa S, Emi S, Tsukui T, Beppu, F.,, Arch. Biochem. Biophys., 504(1), 17, 2010
  19. Maeda H, Hosokawa M, Sashima T, Miyashita K, J. Agric. Food Chem., 55, 7701, 2007
  20. Maeda H, Hosokawa M, Sashima T, Funayama K, Miyashita K, Biochem. Biophys. Res. Commun., 332(2), 392, 2005
  21. Wang WJ, Wang GC, Zhang M, Tseng CK, J. Integr. Plant Biol., 47, 1009, 2005
  22. Kanazawa K, Ozaki Y, Hashimoto T, Das SK, Matsushita S, Hirano M, Okada T, Komoto A, Mori N, Nakatsuka M, Food Sci. Technol. Res., 14, 573, 2008
  23. Rho MK, Uddin MS, Chun BS, Biotechnol. Bioprocess E., 13, 724, 2008
  24. Shang YF, Kim SM, Lee WJ, Um BH, J. Biosci. Bioeng., 111(2), 237, 2011
  25. Lee JY, Kim JH, Korean J. Chem. Eng., 28(7), 1561, 2011