The streaming potential generated by the electrokinetic flow effect within electric double layer of harged channel is applied to determine the membrane potential by using the Helmholtz-Smoluchowski equation. It is known that the membrane potential depends on the particle concentration, solution ionic strength and cake layer formed during the progress of filtration. The influence of physicochemical parameters upon the filtration has been examined with an in-situ and simultaneously monitoring of membrane potential as well as permeate flux. As the latex concentration increases, both permeate flux and membrane potential are decreased. Evidently, the growth of cake layer has been more developed with increasing latex concentration, and then both a lower permeate velocity and a higher solution conductivity lead to decrease the membrane potential. With increasing ionic concentration of KCl from 0.1 to 10 mM, the opposite behavior has been observed, where the permeate flux decreases but the membrane potential increases. Note that the increase of ionic concentration provides a denser cake layer due to the shrinkage of Debye length, and the increased membrane potential results from a corresponding zeta potential.