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Search / Korean Journal of Chemical Engineering
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Korean Chemical Engineering Research, Vol.53, No.6, 831-835, 2015
(+)-Dihydromyricetin 분별침전에 미치는 제타전위의 영향
Influence of Zeta Potential on Fractional Precipitation of (+)-Dihydromyricetin
본 연구에서는 바이오매스 유래 생리활성물질인 (+)-dihydromyricetin을 정제하기 위한 분별침전공정에서 분별침전용액의 제타전위가 분별침전 양상(순도, 수율, 침전물 형태와 크기)에 미치는 영향을 조사하였다. 제타전위 조절을 위한 실리카-알루미나의 첨가량(반응액 부피당 표면적) 100 mm-1에서 가장 높은 수율을 얻을 수 있었다. 실리카-알루미나의 제타전위가 양(+)의 값으로 증가할수록 (+)-dihydromyricetin 수율과 침전물의 크기는 증가하였다. 가장 큰 제타전위 값(+4.99 mV)을 가진 실리카를 이용한 분별침전의 경우에는 가장 작은 제타전위 값(-19.00 mV)을 가진 알루미 나를 이용한 분별침전의 경우보다 2배 이상 높은 수율을 얻을 수 있었다. 또한 분별침전 과정에서 제타전위 절대값이 증가할수록 (+)-dihydromyricetin 수율과 침전물의 크기는 감소하여 제타전위 절대값에 반비례함을 알 수 있었다. 반면 표면적증가물질 실리카-알루미나의 제타전위 변화에도 (+)-dihydromyricetin 순도는 거의 변화가 없었다.
This study evaluated the influence of the zeta potential of silica-alumina on the behavior in terms of purity, yield, and precipitate shape and size of fractional precipitation in the fractional precipitation process for the purification of (+)-dihydromyricetin. The optimal silica-alumina amount (surface area per working volume of reacting solution) for zeta potential control was 100 mm-1. As the zeta potential value of silica-alumina increased, (+)-dihydromyricetin yield and precipitate size were increased. The use of silica with the highest value of the zeta potential (+4.99 mV) as a zeta potential-controlling material increased the (+)-dihydromyricetin yield by 2-fold compared with that of the use of alumina with the lowest value of the zeta potential (-19.00 mV). In addition, the (+)-dihydromyricetin yield and precipitate size was inversely correlated with the absolute value of the zeta potential. On the other hand, the purity of (+)-dihydromyricetin had almost no effect on changes in the zeta potential of silica-alumina.
[References]
- An SW, Kim YG, Kim MH, Lee BI, Lee SH, Kwon HI, Hwang B, Lee HY, Korean J. Medicinal. Crop. Sci., 7, 263, 1999
- Hase K, Basnet P, J. Trad. Med., 14, 28, 1997
- Lee MK, Kim YG, An SW, Kim MH, Lee JH, Lee HY, Korean J. Medicinal. Crop. Sci., 7, 185, 1999
- Sakai K, Yamane T, Saitoh Y, Ikawa C, Nishihata T, Chem Pharm Bull, 35, 4597, 1987
- Yoo SM, Mun S, Kim JH, Process Biochem., 41, 567, 2006
- Du Q, Cai W, Xia M, Ito Y, J. Chromatogr. A, 973, 217, 2002
- Yohsikawa M, Murakami T, Ueda T, Yoshizumi S, Ninomiya K, Murakami N, et al., Yakugaku Zasshi., 117, 108, 1997
- Song X, Ren Q, “Preparation and Application of Dihydromyricetin,” CN. Patent No. 1,288,892(2001).
- Zhang Y, “Process for Preparing Dihydromyricetin from Porcelain Ampelopsis,” CN. Patent No. 1,393,443(2003).
- Lee KH, Kim JH, Biotechnol. Bioeng., 13, 274, 2008
- Lim MK, Kim JH, Biotechnol. Bioeng., 42, 25, 2014
- Han MG, Kim JH, Biotechnol. Bioeng., 17, 1018, 2012
- Ryu HK, Kim JH, Biotechnol. Bioeng., 42, 114, 2014
- Jeon YL, Kim JH, Korean J. Chem. Eng., 30(10), 1954, 2013
- Gregg SJ, Sing KSW, “Adsorption, Surface Area and Porosity,” 2nd ed. Academic Press. New York., pp. 41-110(1982).
- Cho EB, Cho WK, Cha KH, Park JS, Int. J. Pharm., 396, 91, 2010
- Ahmed T, Marcal H, Lawless M, Wanandy NS, Chiu A, Foster LJR, Biomacromolecules, 11(10), 2707, 2010
- Doi Y, Seibunkaisei koubunshi zairyou, Kougyoutyousakai, 1992.
- Jikken kagaku kouza 29 koubunshi zairyou, Nihon kagakukai, 1989.
- Li WJ, Laurencin CT, Caterson EJ, Tuan RS, Ko FK, J. Biomed. Mater. Res., 60, 613, 2002
- Dhandayuthapani B, Krishnan UM, Sethuraman SJ, J. Biomed. Mater. Res., 94, 264, 2010
- Philip S, Keshavarz T, Roy I, J. Chem. Technol. Biotechnol., 82(3), 233, 2007
- Loh XJ, Tan KK, Li X, Li J, Biomaterials, 27, 1841, 2006
- Foster LJR, Sanguanchaipaiwong V, Gabelish CL, Hook J, Stenzel M, Polymer, 46(17), 6587, 2005
- Abe Y, Tsuchida H, Koubunshi to saiboumaku no sougosayou, Kyouritu Syuppan, 1998.
- Ikada Y, Biomaterial nyumon, 1994.
- Zhao K, Deng Y, Chen JC, Chen GQ, Biomaterials, 24, 1041, 2003
- Chen GQ, Wu Q, Biomaterials, 26, 6565, 2005
- Iyou zairyou no kagaku, Nihon kagakukai, 1978.
- Pierschbacher MD, Ruoslahti E, Nature, 309, 30, 1984
- Bhat VD, Truskey GA, Reichert WM, J. Biomed. Mater. Res., 41, 377, 1998
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