| Issue |
Korean Chemical Engineering Research,
Vol.60, No.4, 544-549, 2022
Vol.60, No.4, 544-549, 2022
Taxus chinensis로부터 파클리탁셀 정제를 위한 음압 캐비테이션 아세톤-펜테인 분별침전
Negative Pressure Cavitation Acetone-Pentane Fractional Precipitation for the Purification of Paclitaxel from Taxus chinensis
본 연구에서는 음압 캐비테이션 아세톤-펜테인 분별침전으로 Taxus chinensis로부터 파클리탁셀의 침전 효율을 획기적으로 개선하였다. 음압 -200 mmHg에서 침전할 경우 짧은 조업 시간(5분)에 대부분의 파클리탁셀을 회수(>99.9%) 할 수 있었다. 침전 속도 상수는 대조군 대비 1.512~5.073배(-50~-200 mmHg) 증가하였다. 음압으로 활성화에너지는 -3737~-6536 J/mol 감소하였으며, 이로 인해 침전 속도가 증가하였다. 또한 음압 도입으로 침전물 크기는 5.3배 감소 하였으며, 파클리탁셀의 확산 계수는 7.0배 증가하였다.
This study presents the negative pressure cavitation acetone-pentane fractional precipitation to dramatically improve the precipitation efficiency of paclitaxel from Taxus chinensis. When paclitaxel was precipitated under a negative pressure of -200 mmHg, most paclitaxel (>99.9%) could be recovered in a short precipitation time (5 min). The precipitation rate constant increased by 1.512~5.073 times (at -50 mmHg to -200 mmHg) compared to the control. The activation energy decreased by -3,737~-6,536 J/mol due to negative pressure, which increased the precipitation rate. With the introduction of negative pressure, the precipitate size decreased by 5.3 times, and the diffusion coefficient of paclitaxel increased by 7.0 times.
Keywords:
Paclitaxel; Acetone-pentane fractional precipitation; Negative pressure; Cavitation; Kinetics
[References]
- Montero P, Pérez-Leal M, Pérez-Fidalgo JA, Sanz C, Estornut C, Roger I, Milara J, Cervantes A, Cortijo J, Cancers, 14, 1146, 2022
- Zhu L, Chen L, Cell. Mol. Biol. Lett., 24, 40, 2019
- Caillaud M, Patel NH, White A, Wood M, Contreras KM, Toma W, Alkhlaif Y, Roberts JL, Tran TH, Jackson AB, Poklis J, Gewirtz DA, Damaj MI, Brain. Behav. Immun., 93, 172, 2021
- Bernabeu E, Cagel M, Lagomarsino E, Moretton M, Chiappetta DA, Int. J. Pharm., 526, 474, 2017
- Tan L, Yang LL, Li YJ, Jiang ZF, Li QY, Ma RR, He JY, Zhou LD, Zhang QH, Yuan CS, Microchem J., 165, 106042, 2021
- Jang YS, Kim JH, Biotechnol. Bioprocess Eng., 24, 529, 2019
- Ghorbani M, Pourjafar F, Saffari M, Asgari Y, Meta Gene, 26, 100800, 2020
- Sun T, Liu Y, Li M, Yu H, Piao H, Mol. Cell. Probes, 53, 101602, 2020
- https://www.360researchreports.com/global-paclitaxel-sales-market-16679274.
- Modarresi-Darreh B, Kamali K, Kalantar SM, Dehghanizadeh H, Aflatoonian B, Eurasia J. Biosci., 12, 413, 2018
- Oguzkan SB, Karagul B, Uzun A, Guler OO, Ugras HI, Int. J. Pharmacol., 14, 76, 2018
- Ochoa-Villarreal M, Howat S, Hong S, Jang MO, Jin YW, Lee EK, Loake GJ, BMB Rep., 49, 149, 2016
- Kang HJ, Kim JH, Process Biochem., 99, 316, 2020
- Seo HW, Kim JH, Process Biochem., 87, 238, 2019
- Oh SR, Kim JH, Korean J. Chem. Eng., 38, 480, 2021
- Lee CG, Kim JH, Process Biochem., 59, 216, 2017
- Kang IS, Kim JH, Sep. Purif. Technol., 99, 14, 2012
- Kim JH, Kang IS, Choi HK, Hong SS, Lee HS, Biotechnol. Lett., 22, 1753, 2000
- Kim JH, Kang IS, Choi HK, Hong SS, Lee HS, Process Biochem., 37, 679, 2002
- Jeon SI, Mun S, Kim JH, Process Biochem., 41, 276, 2006
- Jeon YL, Kim JH, Korean J. Chem. Eng., 30, 1954, 2013
- Sim HA, Lee JY, Kim JH, Sep. Purif. Technol., 89, 112, 2012
- Min HS, Kim JH, Biotechnol. Bioprocess Eng., 26, 660, 2021
- Schueller BS, Yang RT, Ind. Eng. Chem. Res., 40, 4912, 2001
- Kang HJ, Kim JH, Biotechnol. Bioprocess Eng., 24, 513, 2019
- Kim JH, Korean Chem. Eng. Res., 58, 273, 2020
- Dalvi SV, Dave RN, Int. J. Pharm., 387, 172, 2010
- Lee SH, Kim JH, Process Biochem., 76, 187, 2019
- Yoo KW, Kim JH, Biotechnol. Bioprocess Eng., 23, 532, 2018
- Min HS, Kim JH, Korean J. Chem. Eng., 39, 58, 2022
- Dalvi SV, Yadav MD, Ultrason. Sonochem., 24, 114, 2015
- Khadka P, Ro J, Kim H, Kim I, Kim JT, Kim H, Cho JM, Yun G, Lee J, Asian J. Pharm. Sci., 9, 304, 2014
- Ma D, Marshall JS, Wu J, J. Acoust. Soc. Am., 114, 3496, 2018
- Guo Z, Jones AG, Li N, Chem. Eng. Sci., 61, 1617, 2008
- Wolloch L, Kost J, J. Control. Release, 148, 204, 2010
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